Markham R B, Yu X, Farzadegan H, Ray S C, Vlahov D
Department of Molecular Microbiology and Immunology, Johns Hopkins School of Hygiene and Public Health, Baltimore, Maryland 21205.
J Infect Dis. 1995 Apr;171(4):797-804. doi: 10.1093/infdis/171.4.797.
Variation in gene sequences from human immunodeficiency virus type 1 (HIV-1) p17gag and the third hypervariable region (V3 loop) of env was examined in a cross-sectional study of single specimens from 4 HIV-1 polymerase chain reaction-positive, seronegative injection drug users. As observed in sexually transmitted disease, clones of HIV-1 genes isolated from an individual were remarkably homogeneous, with amino acid variation in the envelope region of 0.3%-0.9% and in p17gag of 0.0%-0.7%. Intersubject variation was much greater (at the amino acid level, 15% for the envelope and 4.3% for p17gag), with conservation in the envelope only of regions at the base of the V3 loop. Since at least some subjects likely acquired infection intravenously, the ability of virus to survive in seminal fluid or on mucosal surfaces cannot explain the observed homogeneity, which may depend on direct interactions between the virus and the infected cell.
在一项对4名人类免疫缺陷病毒1型（HIV-1）聚合酶链反应阳性、血清学阴性的注射吸毒者的单份标本进行的横断面研究中，检测了HIV-1 p17gag基因序列和env基因第三高变区（V3环）的变异情况。正如在性传播疾病中所观察到的那样，从个体分离出的HIV-1基因克隆非常一致，包膜区域的氨基酸变异为0.3%-0.9%，p17gag的氨基酸变异为0.0%-0.7%。个体间变异要大得多（在氨基酸水平上，包膜为15%，p17gag为4.3%），仅在V3环底部的包膜区域存在保守性。由于至少一些受试者可能是通过静脉注射感染的，病毒在精液或粘膜表面存活的能力无法解释所观察到的一致性，这种一致性可能取决于病毒与受感染细胞之间的直接相互作用。
