Sumyuen M H, Garin Y J, Derouin F
Laboratoire de Parasitologie-Mycologie, UFR Lariboisière-Saint Louis, Université Paris VII, France.
J Parasitol. 1995 Apr;81(2):327-9.
We determined the early kinetics of Toxoplasma gondii infection in Swiss Webster mice inoculated with the avirulent C strain by counting parasites in the blood, spleen, Peyer's patches, liver, lungs, and brain. Animals were orally inoculated with cysts on day zero (D0), and parasites were counted using a tissue culture method at 12, 24, and 36 hr and 2, 3, 7, 10, 14, 21, 30, 50, and 72 days postinfection. The spleen and Peyer's patches were the first organs found parasitized, on D2 and D3, respectively, followed by the lungs and liver on D7 and the brain on D10. No parasitemia was detected. This suggests that early dissemination of this avirulent strain from the intestine into the general circulation occurs essentially via the lymphatic system. Parasites persisted at a high level in the brain during the chronic phase. In the lungs, parasites were no longer detected by D72, while parasite numbers initially declined in the spleen and Peyer's patches but then showed a second peak, possibly due to recirculation of T. gondii. These results suggest that lymphoid organs play a key role in T. gondii dissemination during the acute phase and may also constitute a persistent source of parasite resurgence.
我们通过对感染无毒力C株的瑞士韦伯斯特小鼠的血液、脾脏、派伊尔结、肝脏、肺和脑内的寄生虫进行计数，来测定弓形虫感染的早期动力学。在第0天（D0）给动物经口接种包囊，并在感染后12、24和36小时以及2、3、7、10、14、21、30、50和72天，使用组织培养法对寄生虫进行计数。脾脏和派伊尔结分别是在第2天和第3天首次发现有寄生虫寄生的器官，随后在第7天是肺和肝脏，第10天是脑。未检测到寄生虫血症。这表明这种无毒力株从肠道向全身循环的早期播散基本上是通过淋巴系统发生的。在慢性期，寄生虫在脑中持续处于高水平。在肺中，到第72天不再检测到寄生虫，而在脾脏和派伊尔结中，寄生虫数量最初下降，但随后出现第二个峰值，这可能是由于弓形虫的再循环。这些结果表明，淋巴器官在急性期弓形虫的播散中起关键作用，并且可能也是寄生虫复发的持续来源。
