Miller K G, Kellogg D R
Department of Biology, Washington University, St. Louis, Missouri 63130.
Methods Cell Biol. 1994;44:259-77. doi: 10.1016/s0091-679x(08)60918-8.
In this summary of methods, we have attempted to present a series of protocols relevant to the study of proteins that associate with each other inside cells. These techniques have found their main application, in our hands, to cytoskeletal structures, but the utility of these techniques is limited to this application. As we learn more about the physiology and organization of eukaryotic cells, information from a variety of studies and systems indicates that many processes occur in a highly spatially organized manner in cells, using multiprotein complexes. The techniques described here will find application to the study of how organization and associations between proteins contribute to many of cellular processes.
在本方法总结中，我们试图呈现一系列与细胞内相互关联蛋白质研究相关的实验方案。在我们的研究中，这些技术主要应用于细胞骨架结构，但这些技术的实用性仅限于此应用。随着我们对真核细胞生理学和组织的了解越来越多，来自各种研究和系统的信息表明，许多过程在细胞中以高度空间组织化的方式发生，利用多蛋白复合物。这里描述的技术将应用于研究蛋白质之间的组织和关联如何促成许多细胞过程。
