Takahara T
Department of Surgery, School of Medicine, Keio University, Tokyo, Japan.
Nihon Geka Gakkai Zasshi. 1995 Feb;96(2):59-71.
Chemosensitivity test using growth chamber (GC) was executed on 7 human and murine cell lines, 16 human tumor xenografts and 60 fresh surgical specimens. Dissociated tumor cells suspended in tumor growth medium were plated into GC and incubated with a various concentration of mitomycin C (MMC), adriamycin (ADM), cisplatin (DDP) and 5-fluorouracil (5-FU). After an incubation of 7 days, the activity of hexosaminidase was detected with EIA reader. The antitumor spectra detected by GC assay was essentially identical to those of in vivo nude mouse system, although no evaluable optical density was obtained in normal stromal cell lines. The optimal cutoff concentration of each drug to predict in vivo results was estimated to be 10 micrograms/ml for MMC, 15 micrograms/ml for DDP and 5-FU, and 0.7 microgram/ml for ADM. The predictable accuracy of GC assay on human tumor xenografts was 82.0%. Fifty five clinical specimens were evaluable from 60 cases, and the efficacy rates of MMC, ADM, DDP and 5-FU were 13.3, 25.0, 25.0, and 12.5%, respectively, and the overall accuracy for clinical effects was 72.1%. This assay was thought to be useful for clinical specimens in particular with a large amount of stromal cells.
使用生长室（GC）对7种人源和鼠源细胞系、16个人类肿瘤异种移植瘤以及60份新鲜手术标本进行了化学敏感性测试。将悬浮于肿瘤生长培养基中的解离肿瘤细胞接种到GC中，并与不同浓度的丝裂霉素C（MMC）、阿霉素（ADM）、顺铂（DDP）和5-氟尿嘧啶（5-FU）一起孵育。孵育7天后，用酶免疫分析仪检测己糖胺酶的活性。尽管在正常基质细胞系中未获得可评估的光密度，但通过GC测定法检测到的抗肿瘤谱与体内裸鼠系统的基本相同。预测体内结果的每种药物的最佳截断浓度估计为MMC为10微克/毫升，DDP和5-FU为15微克/毫升，ADM为0.7微克/毫升。GC测定法对人类肿瘤异种移植瘤的预测准确率为82.0%。60例中有55份临床标本可评估，MMC、ADM、DDP和5-FU的有效率分别为13.3%、25.0%、25.0%和12.5%，临床疗效的总体准确率为72.1%。该测定法被认为对特别是含有大量基质细胞的临床标本有用。
