Sakamoto Y, Fujita M, Goi M, Tsukamoto F, Sugimoto T, Okuyama Y, Rhi M, Kusuyama T, Fujita F
Dept. of Surgery, Research Institute for Microbial Diseases, Osaka University, Japan.
Gan To Kagaku Ryoho. 1993 Mar;20(4):447-54.
We assessed the sensitivity to anticancer drugs by SDI (succinate dehydrogenase inhibition) test with 11 clinical specimens obtained from operated patients. The results were compared with those of xenografted specimen in nude mice, using the adjuvant part of the specimens assayed. Chemosensitivity of clinical specimens against 3 drugs is mitomycin (MMC), adriamycin (ADM) and cisplatin (CDDP), showed a good accordance (73%) with those of xenografted tumors. The drug sensitivity was considered to be one of the features of original tumors and to be well preserved in the xenografts in nude mice. We also studied in vivo experimental chemotherapy on 19 tumor lines in nude mice to compare the chemosensitivity with clinical response to the same chemotherapy observed in each donor patient. A close correlation (83% on responder, 100% on non-responder and the overall predicting accuracy rate were 95%) was shown, suggesting in vivo nude mouse assay having an excellent predictability for clinical results.
我们通过琥珀酸脱氢酶抑制（SDI）试验，对11例手术患者的临床标本进行了抗癌药物敏感性评估。使用所检测标本的辅助部分，将结果与裸鼠异种移植标本的结果进行比较。临床标本对丝裂霉素（MMC）、阿霉素（ADM）和顺铂（CDDP）这3种药物的化学敏感性，与异种移植肿瘤的结果显示出良好的一致性（73%）。药物敏感性被认为是原发肿瘤的特征之一，并且在裸鼠异种移植中得到了很好的保留。我们还对19种裸鼠肿瘤细胞系进行了体内实验化疗，以比较化学敏感性与各供体患者对相同化疗的临床反应。结果显示出密切的相关性（反应者为83%，无反应者为100%，总体预测准确率为95%），表明裸鼠体内试验对临床结果具有出色的预测性。
