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[应用原位杂交技术检测慢性前列腺炎中的沙眼衣原体(初步方法学报告)]

[Detection of Chlamydia trachomatis in chronic prostatitis by in situ hybridization (preliminary methodical report)].

作者信息

Kádár A, Bucsek M, Kardos M, Corradi G

机构信息

II. Pathologiai Intézet, Semmelweis Orvostudományi Egyetem, Budapest.

出版信息

Orv Hetil. 1995 Mar 26;136(13):659-62.

PMID:7708396
Abstract

(Preliminary methodical report). In situ hybridization of Chlamydia trachomatis in formalin fixed, paraffin embedded specimens from the Urology Clinic were performed in 2nd Department of Pathology Semmelweis University of Medicine by a biotin labelled DNA probe. Chlamydia trachomatis is suspected to be responsible for the chronic abacterial inflammation of the prostate besides Ureaplasma urealyticum and Mycoplasmae. According to our retrospective study out of 79 biopsy specimens 34 had the diagnosis of chronic abacterial prostatitis. We examined 11 specimens of them. Bacteria were not identified. Three specimens were positive for Chlamydia trachomatis which were improved by transmission electron microscope. The age of patients was between 59 and 81 years. The detection of chlamydia infection rises the suspicion of a "healthy" career and especially at younger age could cause Chlamydia trachomatis associated genital disease. The undiagnosed and untreated diseases may lead to infertility.

摘要

(初步方法学报告)。塞梅尔维斯医科大学病理学第二系使用生物素标记的DNA探针,对泌尿外科诊所福尔马林固定、石蜡包埋标本中的沙眼衣原体进行原位杂交。除解脲脲原体和支原体外,沙眼衣原体被怀疑是前列腺慢性无菌性炎症的病因。根据我们的回顾性研究,在79份活检标本中,有34份被诊断为慢性无菌性前列腺炎。我们检查了其中11份标本。未发现细菌。3份标本沙眼衣原体呈阳性,经透射电子显微镜证实。患者年龄在59至81岁之间。衣原体感染的检测增加了对“健康”职业的怀疑,尤其是在年轻时可能导致沙眼衣原体相关的生殖器疾病。未诊断和未治疗的疾病可能导致不孕。

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