Bach I, Rhodes S J, Pearse R V, Heinzel T, Gloss B, Scully K M, Sawchenko P E, Rosenfeld M G
Eukaryotic Regulatory Biology Program, University of California, San Diego, School of Medicine, La Jolla 92093-0648, USA.
Proc Natl Acad Sci U S A. 1995 Mar 28;92(7):2720-4. doi: 10.1073/pnas.92.7.2720.
A pituitary LIM homeodomain factor, P-Lim, is expressed as Rathke's pouch forms and as specific pituitary cell phenotypes are established, suggesting functional roles throughout pituitary development. While selectively expressed in both anterior and intermediate pituitary in mature mice, P-Lim is also transiently expressed in the developing ventral neural cord and brainstem. P-Lim binds to and activates the promoter of the alpha-glycoprotein subunit gene, a marker of early pituitary development, and synergizes with Pit-1 in transcriptional activation of genes encoding terminal differentiation markers. The LIM domain of P-Lim specifically interacts with the Pit-1 POU domain and is required for synergistic interactions with Pit-1, but not for basal transcriptional activation events.
一种垂体LIM同源结构域因子P-Lim，在拉特克囊形成以及特定垂体细胞表型确立时表达，这表明其在垂体发育全过程中发挥功能作用。在成熟小鼠的垂体前叶和中间叶中均有选择性表达的同时，P-Lim在发育中的腹侧神经索和脑干中也有短暂表达。P-Lim可结合并激活α-糖蛋白亚基基因的启动子，该基因是垂体早期发育的一个标志物，并且在编码终末分化标志物的基因的转录激活过程中与Pit-1协同作用。P-Lim的LIM结构域与Pit-1的POU结构域特异性相互作用，是与Pit-1协同相互作用所必需的，但对于基础转录激活事件并非必需。
