乳腺癌中类固醇受体的检测：酶免疫分析与免疫组化方法之间的关系

Detection of steroid receptors in breast cancer: relationship between EIA and IHC methods.

作者信息

Lerma E, Esqué C, Peiró G, Mora J, Cerdá I, Prat J

机构信息

Department of Pathology, Hospital de la Santa Creu i Sant Pau, Autonomous University of Barcelona, Spain.

出版信息

Scand J Clin Lab Invest. 1994 Dec;54(8):591-4. doi: 10.3109/00365519409087536.

DOI:10.3109/00365519409087536

PMID:7709160

Abstract

The efficiency of EIA and IHC (frozen tissue) in the detection of ER and PR in 51 breast cancers were compared. ER were detected in 51% of cases by IHC and in 49% of tumours by EIA. PR were detected in 56.8% of cases by IHC and in 54.8% of tumours by EIA. Concordance of the results of IHC and EIA reached 78.4% in ER and 82.4% in PR detection. Discordance between IHC and EIA seems to be related to minor changes in sensibility of methods, but we cannot conclude that one of the two techniques is superior to the other in the detection of hormonal receptors. There is a common trend of association between low grade of histologic or nuclear malignancy and a high expression of hormonal receptors.

摘要

比较了酶免疫分析（EIA）和免疫组化（IHC，冰冻组织）检测51例乳腺癌中雌激素受体（ER）和孕激素受体（PR）的效率。免疫组化检测出51%的病例存在ER，酶免疫分析检测出49%的肿瘤存在ER。免疫组化检测出56.8%的病例存在PR，酶免疫分析检测出54.8%的肿瘤存在PR。免疫组化和酶免疫分析结果在ER检测中的一致性达到78.4%，在PR检测中的一致性达到82.4%。免疫组化和酶免疫分析之间的不一致似乎与方法敏感性的微小变化有关，但我们不能得出两种技术中的一种在激素受体检测方面优于另一种的结论。组织学或核恶性程度低与激素受体高表达之间存在共同的关联趋势。