Dono K, Wood M L, Ozato H, Otsu I, Gottschalk R, Maki T, Monaco A P
Department of Surgery, Deaconess Hospital, Boston, MA 02215, USA.
Transplantation. 1995 Apr 15;59(7):929-32. doi: 10.1097/00007890-199504150-00001.
The effect of intrathymic (IT) injection of donor splenocytes and a short course of rapamycin (Rapa) treatment on rat to mouse skin xenograft survival was investigated. ACI rat skin xenografts were transplanted to (C57BL/6 x A)F1 mice treated with rabbit anti-mouse lymphocyte serum (ALS) on days -1, +2, and +4 relative to skin grafting on day 0. Fifty million donor-type splenocytes were injected intrathymically on day 7 after transplantation. Rapa was given intraperitoneally every other day from day 0 to day 12 at a dose of 3.0 mg/kg. Prolonged skin xenograft survival was observed in ALS- and Rapa-treated recipients (no IT injection) with a median survival time of 47 days. However, skin graft survival was markedly more prolonged in the group treated with ALS, Rapa, and IT injection of donor splenocytes did not have a beneficial effect on skin xenograft survival in ALS-treated recipients. An increased presence of donor-type cells was observed in the thymus of the ALS- and Rapa-treated recipients for 7 days after IT injection of donor splenocytes. In conclusion, a short course of Rapa markedly augments rat skin xenograft survival in ALS-treated mice injected intrathymically with donor-type splenocytes.
研究了胸腺内（IT）注射供体脾细胞和短期雷帕霉素（Rapa）治疗对大鼠到小鼠皮肤异种移植存活的影响。将ACI大鼠皮肤异种移植到在相对于第0天皮肤移植的第-1、+2和+4天用兔抗小鼠淋巴细胞血清（ALS）处理的（C57BL/6×A）F1小鼠上。在移植后第7天胸腺内注射五千万个供体型脾细胞。从第0天到第12天每隔一天腹腔注射Rapa，剂量为3.0mg/kg。在接受ALS和Rapa治疗的受体（未进行IT注射）中观察到皮肤异种移植存活时间延长，中位存活时间为47天。然而，在接受ALS、Rapa和IT注射供体脾细胞治疗的组中，皮肤移植存活时间明显延长得更多，而在接受ALS治疗的受体中，IT注射供体脾细胞对皮肤异种移植存活没有有益影响。在IT注射供体脾细胞后7天，在接受ALS和Rapa治疗的受体胸腺中观察到供体型细胞的存在增加。总之，短期Rapa显著提高了在胸腺内注射供体型脾细胞的接受ALS治疗的小鼠中大鼠皮肤异种移植的存活时间。
