Konstantinova M F, Bogomazova A N, Beliaeva T N, Bulychev A G, Leont'eva E A
Tsitologiia. 1994;36(11):1113-7.
It has been shown by electrophoresis that the earlier obtained thermoresistant sublines of the CHO-K1 cell line do not accumulate heat shock proteins when cultured at 37 degrees C. The thermostability of two lysosomal proteins--acid lipase and acid phosphatase--were higher in the thermoresistant cells than in the parental cells, whereas no differences in thermostability of galactosidase were found between heat resistant and parental lines. Thus, it is concluded that changes in the level of conformational flexibility of protein molecules may be one of the mechanisms of cell adaptation to growth at higher temperatures.
电泳结果表明，早期获得的CHO-K1细胞系耐热亚系在37℃培养时不会积累热休克蛋白。两种溶酶体蛋白——酸性脂肪酶和酸性磷酸酶——在耐热细胞中的热稳定性高于亲代细胞，而耐热细胞系和原代细胞系之间半乳糖苷酶的热稳定性没有差异。因此，可以得出结论，蛋白质分子构象灵活性水平的变化可能是细胞适应在较高温度下生长的机制之一。
