克氏锥虫：主要半胱氨酰蛋白酶（克氏锥虫蛋白酶）与寄生虫胞质酸性抗原（FIV）的免疫交叉反应性。

Trypanosoma cruzi: immunological cross-reactivity of the major cysteinyl proteinase (cruzipain) with a parasite cytosol acidic antigen (FIV).

作者信息

Cerban F M, Motran C, Laderach D, Gea S, Gruppi A, Vottero-Cima E

机构信息

Departamento de Bioquimica Clinica, Facultad de Ciencias Quimicas, Universidad Nacional de Cordoba, Ciudad Universitaria, Pabellon Argentina.

出版信息

Acta Trop. 1994 Dec;58(3-4):337-43. doi: 10.1016/0001-706x(94)90027-2.

DOI:10.1016/0001-706x(94)90027-2

PMID:7709872

Abstract

This paper shows that a polyclonal monospecific rabbit antiserum to cruzipain, the major T. cruzi cystein proteinase, cross-reacts with a cytosol acidic antigen (F IV) isolated from the epimastigote stage of the same parasite. In addition, antibodies specific for F IV purified from chagasic patient sera or murine anti F IV sera, also react with cruzipain. This was demonstrated by ELISA, DOT-ELISA, native and electrophoretic Immunoblot. These findings suggest that F IV contains an antigen immunologically cross-reactive with cruzipain.

摘要

本文表明，针对克氏锥虫主要半胱氨酸蛋白酶克氏锥虫蛋白酶的多克隆单特异性兔抗血清，与从同一寄生虫的上鞭毛体阶段分离出的一种胞质酸性抗原（F IV）发生交叉反应。此外，从恰加斯病患者血清中纯化的针对F IV的抗体或鼠抗F IV血清，也与克氏锥虫蛋白酶发生反应。这通过酶联免疫吸附测定（ELISA）、斑点酶联免疫吸附测定（DOT-ELISA）、天然免疫印迹和电泳免疫印迹得以证实。这些发现表明，F IV含有一种与克氏锥虫蛋白酶发生免疫交叉反应的抗原。