de la Hoz F, Melero J A, González R, Carreira J
Alergia e Inmunología Abelló SA, Madrid, Spain.
Allergy. 1994 Dec;49(10):848-54. doi: 10.1111/j.1398-9995.1994.tb00786.x.
We have purified four allergens from Helianthus annuus (sunflower) pollen, hereafter named as allergens a, b, c, and d. Under native conditions, allergen a has a mol. mass of 32, allergen b has one of 24, and allergens c and d each have one of 55 kDa. At the least, allergens b, c, and d demonstrate charge heterogeneity, and the electrophoretic mobility of allergens c and d increases when these allergens are deglycosylated with trifluoromethanesulfonic acid. Cross-reactivity among the four allergens and with the whole extract is very high, and each allergen recognizes IgE in a high proportion of patients sensitized to sunflower pollen.
我们已从向日葵花粉中纯化出四种过敏原，以下分别命名为过敏原a、b、c和d。在天然条件下，过敏原a的分子量为32 kDa，过敏原b为24 kDa，过敏原c和d均为55 kDa。至少过敏原b、c和d表现出电荷异质性，用三氟甲磺酸对过敏原c和d进行去糖基化处理后，它们的电泳迁移率会增加。这四种过敏原之间以及与整个提取物之间的交叉反应性非常高，并且每种过敏原在很大比例的对向日葵花粉过敏的患者中都能识别IgE。
