• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Detection of giant myofibrillar proteins connectin and nebulin by electrophoresis in 2% polyacrylamide slab gels strengthened with agarose.

作者信息

Tatsumi R, Hattori A

机构信息

Department of Animal Science, Faculty of Agriculture, Hokkaido University, Japan.

出版信息

Anal Biochem. 1995 Jan 1;224(1):28-31. doi: 10.1006/abio.1995.1004.

DOI:10.1006/abio.1995.1004
PMID:7710083
Abstract

We have established an improved method of sodium dodecyl sulfate-polyacrylamide gel electrophoresis to facilitate analysis of giant myofibrillar proteins connectin and nebulin, whose molecular masses are about 3000 and 700 kDa, respectively. This method consists of 2% polyacrylamide slab gels strengthened with agarose and a specific buffer system. Our 2% polyacrylamide slab gels are superior to usual 1.8 or 2% polyacrylamide disc gels in preparation, handling, and resolution.

摘要

相似文献

1
Detection of giant myofibrillar proteins connectin and nebulin by electrophoresis in 2% polyacrylamide slab gels strengthened with agarose.
Anal Biochem. 1995 Jan 1;224(1):28-31. doi: 10.1006/abio.1995.1004.
2
Detection of giant myofibrillar proteins connectin and nebulin in fish meat by electrophoresis in 3-5 gradient sodium dodecyl sulfate polyacrylamide slab gels.通过在3-5梯度十二烷基硫酸钠聚丙烯酰胺平板凝胶中进行电泳检测鱼肉中的巨大肌原纤维蛋白连接蛋白和伴肌动蛋白。
J Agric Food Chem. 2002 Dec 18;50(26):7499-503. doi: 10.1021/jf020362l.
3
Gel electrophoresis of giant proteins: solubilization and silver-staining of titin and nebulin from single muscle fiber segments.巨大蛋白质的凝胶电泳：来自单个肌纤维节段的肌联蛋白和伴肌动蛋白的溶解及银染
Electrophoresis. 1993 Jan-Feb;14(1-2):56-64. doi: 10.1002/elps.1150140110.
4
Detection of myofibrillar proteins using a step gradient minigel with an ambiguous interface.
Anal Biochem. 2005 Mar 15;338(2):270-7. doi: 10.1016/j.ab.2004.12.022.
5
The resolution between two native proteins and between their sodium dodecyl sulfate-complexes in agarose and polyacrylamide gel electrophoresis.两种天然蛋白质之间以及它们在琼脂糖和聚丙烯酰胺凝胶电泳中的十二烷基硫酸钠复合物之间的分辨率。
Electrophoresis. 1997 Jun;18(7):1126-32. doi: 10.1002/elps.1150180717.
6
Vertical agarose gel electrophoresis and electroblotting of high-molecular-weight proteins.高分子量蛋白质的垂直琼脂糖凝胶电泳和电印迹
Electrophoresis. 2003 Jun;24(11):1695-702. doi: 10.1002/elps.200305392.
7
Connectin content in rabbit cardiac and skeletal muscle.兔心肌和骨骼肌中的连接蛋白含量
Int J Biochem. 1993 Dec;25(12):1853-8.
8
Ultrathin-layer sodium dodecyl sulfate disc electrophoresis of proteins in the range from 10 to 220 kDa in homogeneous, low-concentrated polyacrylamide gels.在均匀、低浓度聚丙烯酰胺凝胶中对分子量范围为10至220 kDa的蛋白质进行超薄层十二烷基硫酸钠圆盘电泳。
Electrophoresis. 2003 Jul;24(14):2272-6. doi: 10.1002/elps.200305499.
9
Changes in the molecular types of connectin and nebulin during development of chicken skeletal muscle.
Biochim Biophys Acta. 1995 May 11;1244(1):179-84. doi: 10.1016/0304-4165(94)00224-l.
10
[Improved detection of macromolecular proteins with sodium dodecyl sulfate-polyacrylamide slab gel electrophoresis].[用十二烷基硫酸钠-聚丙烯酰胺平板凝胶电泳改进大分子蛋白质的检测]
Seikagaku. 1989 Aug;61(8):717-9.

引用本文的文献

1
Sacubitril/valsartan improves diastolic left ventricular stiffness with increased titin phosphorylation via cGMP-PKG activation in diabetic mice.沙库巴曲缬沙坦通过 cGMP-PKG 激活增加肌联蛋白磷酸化改善糖尿病小鼠舒张期左心室僵硬度。
Sci Rep. 2024 Oct 23;14(1):25081. doi: 10.1038/s41598-024-75757-8.
2
Alterations in cardiac contractile and regulatory proteins contribute to age-related cardiac dysfunction in male rats.心脏收缩和调节蛋白的改变导致雄性大鼠与年龄相关的心脏功能障碍。
Physiol Rep. 2024 Aug;12(16):e70012. doi: 10.14814/phy2.70012.
3
Urinary titin is not an early biomarker of skeletal muscle atrophy induced by muscle denervation in mice.
尿中的 titin 不是由肌肉失神经支配引起的小鼠骨骼肌萎缩的早期生物标志物。
PLoS One. 2023 Aug 15;18(8):e0289185. doi: 10.1371/journal.pone.0289185. eCollection 2023.
4
Changes in the Mechanical Properties of Fast and Slow Skeletal Muscle after 7 and 21 Days of Restricted Activity in Rats.限制大鼠活动 7 和 21 天后快速和慢速骨骼肌力学性能的变化。
Int J Mol Sci. 2023 Feb 18;24(4):4141. doi: 10.3390/ijms24044141.
5
Calpain-dependent degradation of cytoskeletal proteins as a key mechanism for a reduction in intrinsic passive stiffness of unloaded rat postural muscle.钙蛋白酶依赖性细胞骨架蛋白降解是未负荷大鼠姿势肌内在被动僵硬降低的关键机制。
Pflugers Arch. 2022 Nov;474(11):1171-1183. doi: 10.1007/s00424-022-02740-5. Epub 2022 Aug 6.
6
Predominant synthesis of giant myofibrillar proteins in striated muscles of the long-tailed ground squirrel Urocitellus undulatus during interbout arousal.在长尾巴地松鼠 Urocitellus undulatus 的觉醒间歇期，横纹肌中巨肌原纤维蛋白的主要合成。
Sci Rep. 2020 Sep 16;10(1):15185. doi: 10.1038/s41598-020-72127-y.
7
High-yield skeletal muscle protein recovery from TRIzol after RNA and DNA extraction.从 TRIzol 中提取 RNA 和 DNA 后，可高效回收骨骼肌蛋白质。
Biotechniques. 2020 Oct;69(4):264-269. doi: 10.2144/btn-2020-0083. Epub 2020 Aug 11.
8
Effect of L-Arginine on Titin Expression in Rat Soleus Muscle After Hindlimb Unloading.L-精氨酸对大鼠后肢卸载后比目鱼肌肌联蛋白表达的影响。
Front Physiol. 2019 Sep 20;10:1221. doi: 10.3389/fphys.2019.01221. eCollection 2019.
9
Abnormal contractility in human heart myofibrils from patients with dilated cardiomyopathy due to mutations in TTN and contractile protein genes.扩张型心肌病患者心肌成纤维细胞肌球蛋白突变及收缩蛋白基因异常导致心肌收缩力异常。
Sci Rep. 2017 Nov 1;7(1):14829. doi: 10.1038/s41598-017-13675-8.
10
Impaired activity of adherens junctions contributes to endothelial dilator dysfunction in ageing rat arteries.黏附连接活性受损导致衰老大鼠动脉内皮舒张功能障碍。
J Physiol. 2017 Aug 1;595(15):5143-5158. doi: 10.1113/JP274189. Epub 2017 Jun 30.