Lerma E, Lee S J, Tugues D, Oliva E, Gich I, Prat J
Department of Pathology, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain.
Anal Quant Cytol Histol. 1994 Dec;16(6):435-40.
DNA ploidy was investigated by flow cytometry (FC) and image analysis (IA) in paraffin-embedded tissue sections from 36 stromal tumors of the gastrointestinal tract. The results of both techniques were correlated with pathologic features of the tumors and survival. Ten (27.8%) tumors were aneuploid by FC and IA. Most of the diploid tumors were identified by both techniques, but FC appeared to be superior to tissue section IA for identification of aneuploid tumors (25% vs. 13.8%). Aneuploidy by FC correlated with pathologic grade and mitotic index (P < .05), and a trend to short survival was also detected (P < .1). No similar correlation was found by IA. Enlargement and variation of nuclei may explain the discrepancy between FC and IA.
通过流式细胞术（FC）和图像分析（IA）对36例胃肠道间质瘤石蜡包埋组织切片中的DNA倍性进行了研究。两种技术的结果均与肿瘤的病理特征及生存情况相关。FC和IA检测显示10例（27.8%）肿瘤为非整倍体。大多数二倍体肿瘤可通过两种技术识别，但FC在识别非整倍体肿瘤方面似乎优于组织切片IA（25%对13.8%）。FC检测的非整倍体与病理分级和有丝分裂指数相关（P<0.05），并且还检测到生存时间缩短的趋势（P<0.1）。IA未发现类似的相关性。细胞核的增大和变异可能解释了FC和IA之间的差异。
