Sultana G N, Watanabe Y, Tamai Y
Department of Bioresources, Faculty of Agriculture, Ehime University, Japan.
Biotechnol Appl Biochem. 1995 Feb;21(1):101-10.
Structural requirements of inorganic phosphorus compounds as specific activators or inhibitors for phospholipase A2 and phospholipase B were investigated using orthophosphate, pyrophosphate and polyphosphate. It was observed that orthophosphate and pyrophosphate stimulated the activities of phospholipase A2 from bee venom, snake (Naja naja) venom and pig pancreas, and also phospholipase B from the yeast Torulaspora delbrueckii. However, polyphosphate was found to act as an inhibitor for phospholipase A2 in the above species and also for phospholipase B from T. delbrueckii. Orthophosphate and pyrophosphate induced gradual aggregation of liposome, but polyphosphate prolonged the lifetime of the liposome, suggesting that orthophosphate and pyrophosphate destabilize the bilayer structure of phosphatidylcholine and polyphosphate stabilizes it.
使用正磷酸盐、焦磷酸盐和多磷酸盐研究了无机磷化合物作为磷脂酶A2和磷脂酶B的特异性激活剂或抑制剂的结构要求。观察到正磷酸盐和焦磷酸盐刺激了来自蜂毒、蛇（眼镜蛇）毒和猪胰腺的磷脂酶A2的活性，以及来自德氏有孢圆酵母的磷脂酶B的活性。然而，发现多磷酸盐对上述物种中的磷脂酶A2以及德氏有孢圆酵母的磷脂酶B起抑制剂作用。正磷酸盐和焦磷酸盐诱导脂质体逐渐聚集，但多磷酸盐延长了脂质体的寿命，这表明正磷酸盐和焦磷酸盐会破坏磷脂酰胆碱的双层结构，而多磷酸盐会使其稳定。
