Whalen J D, Dufresne R G, Wilkel C S
Brown University School of Medicine, Department of Dermatology, Roger Williams Medical Center, Providence, Rhode Island, USA.
Dermatol Surg. 1995 Mar;21(3):241-2. doi: 10.1111/j.1524-4725.1995.tb00162.x.
Toxic exposure to xylene may occur during routine histopathologic staining procedures. Safer xylene substitutes have been available for over a decade, however, a 1991 survey of 25 Mohs training programs revealed that only 56% were using xylene substitutes.
The purpose of this study was to compared xylene to xylene substitutes in the histologic preparation of frozen sections with respect to staining quality, clarity, cellular detail, and tissue distortion.
Xylene and xylene substitutes were used as the final clearing agent in the routine histologic preparation of fresh frozen horizontal sections. The slides were evaluated by a dermatopathologist and a Mohs surgeon in a blinded manner.
No difference between xylene and the xylene substitutes were noted.
In light of the improved safety profile and efficacy of the xylene substitutes, they should replace xylene in the Mohs laboratory.
在常规组织病理学染色过程中可能会发生二甲苯中毒暴露。更安全的二甲苯替代品已经出现十多年了，然而，1991年对25个莫氏手术培训项目的调查显示，只有56%的项目在使用二甲苯替代品。
本研究的目的是在冷冻切片的组织学制备中，就染色质量、清晰度、细胞细节和组织变形方面，将二甲苯与二甲苯替代品进行比较。
在新鲜冷冻水平切片的常规组织学制备中，使用二甲苯和二甲苯替代品作为最后的透明剂。由一名皮肤病理学家和一名莫氏外科医生以盲法对玻片进行评估。
未发现二甲苯与二甲苯替代品之间存在差异。
鉴于二甲苯替代品安全性和有效性的提高，它们应在莫氏实验室中取代二甲苯。
