Raghupathi R, Welsh F A, Lowenstein D H, Gennarelli T A, McIntosh T K
Department of Surgery, University of Pennsylvania, Philadelphia 19104, USA.
J Cereb Blood Flow Metab. 1995 May;15(3):467-73. doi: 10.1038/jcbfm.1995.58.
To evaluate the cellular response to traumatic brain injury, the expression of mRNA for c-fos and the 72-kDa heat shock protein (hsp72) was determined using in situ hybridization following lateral fluid-percussion injury (2.2-2.4 atm) in rat brain. At 2 h after injury, induction of c-fos mRNA was observed throughout the cortex ipsilateral to the site of injury, while increased expression of hsp72 mRNA was restricted to regions of the cortex surrounding the contusion area. An increase in c-fos mRNA, but not hsp72 mRNA, was observed bilaterally in the CA3 subfield of the hippocampus and the granule cells of the dentate gyrus and in the thalamus ipsilateral to the impact site. By 6 h, increased expression of c-fos mRNA was observed only in the corpus callosum on the impact side; hsp72 mRNA persisted in the deep cortical layers and upper layers of the subcortical white matter below the site of maximal injury. By 24 h, both c-fos and hsp72 mRNA had returned to control levels in all regions of the brain. These results demonstrate that lateral fluid-percussion brain injury triggers regionally and temporally specific expression of c-fos and hsp72 mRNA, which may be suggestive of differential neurochemical alterations in neurons and glia following experimental brain injury.
为评估细胞对创伤性脑损伤的反应，在大鼠脑侧方液压冲击伤（2.2 - 2.4大气压）后，采用原位杂交法测定c - fos和72 kDa热休克蛋白（hsp72）的mRNA表达。损伤后2小时，在损伤部位同侧的整个皮质中观察到c - fos mRNA的诱导，而hsp72 mRNA表达增加仅限于挫伤区域周围的皮质区域。在海马CA3亚区、齿状回颗粒细胞以及撞击部位同侧的丘脑中，双侧均观察到c - fos mRNA增加，但hsp72 mRNA未增加。到6小时时，仅在撞击侧的胼胝体中观察到c - fos mRNA表达增加；hsp72 mRNA在最大损伤部位下方的皮质深层和皮质下白质上层持续存在。到24小时时，大脑所有区域的c - fos和hsp72 mRNA均恢复到对照水平。这些结果表明，侧方液压冲击性脑损伤触发了c - fos和hsp72 mRNA在区域和时间上的特异性表达，这可能提示实验性脑损伤后神经元和胶质细胞中存在不同的神经化学改变。
