用于口蹄疫病毒检测与分型的快速协同凝集试验。

Rapid coagglutination test for the detection and typing of foot and mouth disease virus.

作者信息

Montassier H J, Araújo Júnior J P, Pinto A A

机构信息

Faculdade de Ciências Agrárias e Veterinárias da Universidade Estadual Paulista, Jaboticabal-SP, Brazil.

出版信息

J Virol Methods. 1994 Dec;50(1-3):29-41. doi: 10.1016/0166-0934(94)90161-9.

DOI:10.1016/0166-0934(94)90161-9

PMID:7714052

Abstract

Protein A containing Staphylococcus aureus was used to develop a coagglutination (COA) test for the detection and typing of foot and mouth disease virus (FMDV) O, A and C serotypes in infected cells and tissues. Different batches and amounts of guinea pig anti-FMDV sera were assessed to optimize the preparation of COA conjugates. The sensitivity and specificity of the COA Test for the detection of FMDV O, A and C serotypes and heterologous viruses was also characterized. Comparison between the COA Test and complement fixation test for the detection and typing of FMDV obtained from extracts of tongue epithelial tissues from infected cattle revealed high agreement in the results and indicated a potential application of the COA Test for the direct diagnosis of viruses.

摘要

含有金黄色葡萄球菌A蛋白的物质被用于开发一种协同凝集（COA）试验，用于检测和分型感染细胞及组织中的口蹄疫病毒（FMDV）O、A和C血清型。评估了不同批次和数量的豚鼠抗FMDV血清，以优化COA结合物的制备。还对检测FMDV O、A和C血清型及异源病毒的COA试验的敏感性和特异性进行了表征。对从感染牛的舌上皮组织提取物中检测和分型FMDV的COA试验与补体结合试验进行比较，结果显示高度一致，表明COA试验在病毒直接诊断方面具有潜在应用价值。