Yoshimura I, Kudoh J, Saito S, Tazaki H, Shimizu N
Department of Urology, Keio University School of Medicine, Tokyo, Japan.
J Urol. 1995 May;153(5):1711-5.
Surgical specimens from 30 patients with transitional cell carcinoma of the bladder were examined for the presence of mutation of the p53 tumor suppressor gene using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) analysis. All of the specimens were superficial, low grade cancer, and 10 patients had recurrences during an observation period of 24 months following the initial surgical treatment. Among the 30 patients with superficial bladder cancer, 4 were found to have the mutated p53 gene and 3 of them had recurrences, which involved either invasive or high grade tumors. Two of these recurrent tumors with the p53 gene mutation showed identical mutations with those primary tumors, whereas the other one did not have the same mutation. The data suggest that bladder cancers with a mutation of the p53 gene have a greater probability of poor prognosis than those which do not, even if the primary lesion was a superficial, low grade tumor. Therefore, the presence of the p53 gene mutation may provide important clues to the factors involved in bladder cancer.
利用聚合酶链反应-单链构象多态性（PCR-SSCP）分析，对30例膀胱移行细胞癌患者的手术标本进行p53肿瘤抑制基因突变检测。所有标本均为表浅、低级别癌，10例患者在初次手术治疗后的24个月观察期内出现复发。在30例表浅膀胱癌患者中，4例发现有p53基因突变，其中3例出现复发，复发肿瘤为浸润性或高级别肿瘤。这3例伴有p53基因突变的复发性肿瘤中，2例与原发肿瘤显示相同的突变，而另1例则没有相同的突变。数据表明，即使原发病变为表浅、低级别肿瘤，伴有p53基因突变的膀胱癌预后不良的可能性也高于无该突变者。因此，p53基因突变的存在可能为膀胱癌相关因素提供重要线索。
