Neuroanatomical tract tracing provides histological verification of neuron loss following cytotoxic lesions.

In the present study we report a neuroanatomical procedure that provides direct histological verification of the extent of neuron loss following cytotoxic lesions of the lateral subdivision of the habenular complex (Lhb). Following kainic acid-induced lesions of Lhb neurons, the fluorescent retrograde tracer Fluoro-Gold was injected into the ventral midbrain where many medial habenula (Mhb) and Lhb neurons project. The absence of retrogradely labeled neurons in the Lhb indicated the extent of neuron loss there, and the presence of Fluoro-Gold-labeled neurons in the Mhb indicated that its neurons were intact. The extent of neuron loss in the Lhb was significantly correlated with behavioral data. Retrograde tract tracing can be used as an effective histological tool to verify the extent of neuron loss following a lesion procedure.