Bielavska E, Krivanek J
Institute of Physiology, Academy of Sciences, Praha, Czech Republic.
Neurosci Lett. 1994 Dec 5;182(2):239-42. doi: 10.1016/0304-3940(94)90806-0.
The contribution of protein kinase C (PKC) to the acquisition of conditioned taste aversion (CTA) was tested by injection of three PKC inhibitors--polymyxin B, H7 and staurosporine--into the parabrachial nucleus (PBN). From the tested drugs only polymyxin B (20 mM) prevented CTA acquisition. Application of H7 (10 mM) and staurosporine (100 and 500 microM) into the PBN did not impair CTA learning. The blocking effect of polymyxin B is dose dependent (5 and 10 mM concentration did not disrupt CTA formation) and site specific (application of polymyxin B into the visual cortex did not elicit CTA blockade). The ability of polymyxin B to disrupt CTA learning is not due to irreversible damage of PBN. These results suggest that polymyxin B blocks acquisition of CTA in some nonspecific way not necessarily involving inhibition of PKC. This conclusion is supported by failure of two other more specific PKC inhibitors to affect CTA learning.
通过向臂旁核（PBN）注射三种蛋白激酶C（PKC）抑制剂——多粘菌素B、H7和星形孢菌素，来测试PKC对条件性味觉厌恶（CTA）形成的作用。在所测试的药物中，只有多粘菌素B（20 mM）能阻止CTA的形成。向PBN注射H7（10 mM）和星形孢菌素（100和500 microM）并不损害CTA学习。多粘菌素B的阻断作用具有剂量依赖性（5和10 mM浓度不会破坏CTA的形成）且具有位点特异性（将多粘菌素B注射到视觉皮层不会引起CTA阻断）。多粘菌素B破坏CTA学习的能力并非由于PBN的不可逆损伤。这些结果表明，多粘菌素B以某种不一定涉及抑制PKC的非特异性方式阻断CTA的形成。另外两种更具特异性的PKC抑制剂未能影响CTA学习，这一结论得到了支持。
