Ultrastructural immunolocalization of muscarinic acetylcholine receptor in the dorsal thalamus of rat.

The ultrastructural distribution of muscarinic acetylcholine receptor (mAChR) in the dorsal thalamus of the adult rat was studied by means of pre-embedding immunocytochemistry using the monoclonal antibody M35. mAChR immunoreactivity (ir) was present with variable intensity in the different thalamic nuclei, but with a similar subcellular localization. Labeling was restricted to neuronal cell bodies and dendrites, where it was both in the cytoplasm and along the cytoplasmic side of the plasma membrane, in areas post-synaptic to small terminals with round clear vesicles but also in non-synaptic areas. Glial cells were unlabeled. By combining the pre-embedding immunostaining for mAChR with post-embedding immunogold labeling for GABA it was shown that GABAergic terminals made synaptic contacts with cholinoceptive structures, but no mAChR ir was present at their post-synaptic sites.