Berwert L, Segner H, Reinecke M
Institute of Anatomy, University of Zürich, Switzerland.
Peptides. 1995;16(1):113-22. doi: 10.1016/0196-9781(94)00161-x.
The ontogeny of the endocrine pancreas of a teleost, the turbot (Scophthalmus maximus), was investigated by the use of double immunofluorescence. Clustered insulin (INS)-immunoreactive (IR) cells were observed on the first day after hatching. During the following days, the islet largely increased in size and some smaller islets appeared. All islets consisted only of INS-IR cells. Between day 5 (onset of exogenous feeding) and 7, somatostatin (SOM) and glucagon (GLUC) cells appeared. In the large (principal) islet, the SOM-IR cells intermingled with the INS-IR cells. In the secondary islets, they occurred at the periphery. The GLUC-IR cells were located at the periphery in all islets. Subsequently, two-four additional small principal islets appeared. At day 11, pancreatic polypeptide (PP)-IR cells were present in principal islets and secondary islets. Starting with day 11, in all islets, insulin-like growth factor 1 (IGF-1) immunoreactivity was localized in numerous PP-IR cells and GLUC-IR and some SOM-IR cells. It also occurred in enteroendocrine cells that seemed to contain none of the classical islet hormones. The early appearance of INS correlates with its key role in the regulation of fish protein and lipid metabolism. Islet-derived IGF-1 might inhibit the regulation of INS secretion in a paracrine manner and may be highly involved in growth-promoting processes.
利用双重免疫荧光技术研究了硬骨鱼大菱鲆（Scophthalmus maximus）内分泌胰腺的个体发育过程。孵化后第一天就观察到了聚集的胰岛素（INS）免疫反应性（IR）细胞。在接下来的几天里，胰岛体积大幅增大，并且出现了一些较小的胰岛。所有胰岛仅由INS-IR细胞组成。在第5天（开始外源投喂）至第7天之间，生长抑素（SOM）和胰高血糖素（GLUC）细胞出现。在大（主要）胰岛中，SOM-IR细胞与INS-IR细胞相互交织。在次级胰岛中，它们出现在周边。GLUC-IR细胞位于所有胰岛的周边。随后，又出现了两到四个额外的小主胰岛。在第11天，胰腺多肽（PP）-IR细胞出现在主胰岛和次级胰岛中。从第11天开始，在所有胰岛中，胰岛素样生长因子1（IGF-1）免疫反应性定位于众多PP-IR细胞、GLUC-IR细胞和一些SOM-IR细胞中。它也出现在似乎不含任何经典胰岛激素的肠内分泌细胞中。INS的早期出现与其在鱼类蛋白质和脂质代谢调节中的关键作用相关。胰岛来源的IGF-1可能以旁分泌方式抑制INS分泌的调节，并且可能高度参与生长促进过程。
