Thompson K S, Rayne R C, Gibbon C R, May S T, Patel M, Coast G M, Bacon J P
Sussex Centre for Neuroscience, School of Biological Sciences, Brighton, UK.
Peptides. 1995;16(1):95-104. doi: 10.1016/0196-9781(94)00158-3.
Locust abdominal ganglia are shown to colocalize Locusta-diuretic peptide-, leucokinin I-, and lysine vasopressin-like immunoreactivity in posterior lateral neurosecretory cells. Extracts of abdominal ganglia were partially purified by RP-HPLC then dot immunoassay screened with the same antisera used for immunocytochemistry. Locusta-diuretic peptide-like immunoreactive material coeluted with synthetic Locusta-diuretic peptide, and leucokinin-like immunoreactive material coeluted with locustakinin. Lysine vasopressin-like material eluted in fractions that also showed Locusta-diuretic peptide and leucokinin I immunoreactivity. The diuretic activity of synthetic Locusta-diuretic peptide and locustakinin is demonstrated, and they are shown to act at least additively to promote Malpighian tubule fluid secretion. The immunoreactive neurosecretory cells are assumed to express at least these two peptides, and a model for promoting fluid secretion is proposed.
已证明蝗虫腹神经节中，后外侧神经分泌细胞共定位有蝗虫利尿肽、亮氨酸激肽I和赖氨酸加压素样免疫反应性。腹神经节提取物经反相高效液相色谱（RP-HPLC）部分纯化，然后用与免疫细胞化学相同的抗血清进行斑点免疫测定筛选。蝗虫利尿肽样免疫反应性物质与合成的蝗虫利尿肽共洗脱，亮氨酸激肽样免疫反应性物质与蝗虫激肽共洗脱。赖氨酸加压素样物质在也显示出蝗虫利尿肽和亮氨酸激肽I免疫反应性的组分中洗脱。合成的蝗虫利尿肽和蝗虫激肽的利尿活性得到证实，并且它们至少以相加的方式起作用以促进马氏管液分泌。假定免疫反应性神经分泌细胞至少表达这两种肽，并提出了促进液体分泌的模型。
