牛主动脉内皮细胞单层对血栓素A2类似物反应时通透性增加。

Increased permeability of bovine aortic endothelial cell monolayers in response to a thromboxane A2-mimetic.

作者信息

Teixeira C F, Farmer P, Laporte J, Jancar S, Sirois P

机构信息

Lab. Pharmacology, Instituto Butantan, Universidade de São Paulo, Brasil.

出版信息

Agents Actions Suppl. 1995;45:47-52. doi: 10.1007/978-3-0348-7346-8_7.

DOI:10.1007/978-3-0348-7346-8_7

PMID:7717200

Abstract

The present study was designed to investigate the ability of thromboxane to modulate the clearance rate of 125I-albumin through bovine aortic endothelial cell (BAEC) monolayer grown on polycarbonate micropore membrane. Stimulation of BAEC with the TXA2 mimetic U44069 (10(-8), 10(-7) and 10(-6) M) elicited a dose-dependent increase of labeled albumin passage across BAEC monolayers. This effect was markedly reduced by the TXA2 antagonist L655240 (10(-7) and 10(-6) M). Our results suggest that TXA2 may modulate the permeability of endothelial cells directly through activation of specific receptors.

摘要

本研究旨在探讨血栓素通过生长在聚碳酸酯微孔膜上的牛主动脉内皮细胞（BAEC）单层调节125I-白蛋白清除率的能力。用TXA2模拟物U44069（10^(-8)、10^(-7)和10^(-6)M）刺激BAEC，导致标记白蛋白穿过BAEC单层的通量呈剂量依赖性增加。TXA2拮抗剂L655240（10^(-7)和10^(-6)M）可显著降低这种效应。我们的结果表明，TXA2可能通过激活特定受体直接调节内皮细胞的通透性。