Takagishi T, Sasaguri Y, Nakano R, Arima N, Tanimoto A, Fukui H, Morimatsu M
Department of Pathology, Kurume University School of Medicine, Japan.
Am J Pathol. 1995 Apr;146(4):981-8.
Histamine in serum and arterial tissue contributes to the pathogenesis of atherosclerosis and the formation of coronary artery vasospasm. As the effect of histamine at a given site will be mediated by its specific receptors, we investigated by in situ hybridization and Northern blot analysis the expression and localization of human histamine H1 receptor mRNA in the arterial wall and in cultured human aortic intimal smooth muscle cells (SMC) and immortalized SMC (ISS10) and endothelial cells (SE1). In situ hybridization showed that SMC and endothelial cells expressed H1 receptor mRNA in vivo and that the expression was increased in SMC in the thickened intima of atherosclerotic foci in both the aorta and coronary artery. By Northern blot analysis, we also detected histamine H1 receptor mRNA in cultured SMC, ISS10, and SE1 and found that platelet-derived growth factor stimulated SMC to increase their expression of the mRNA in vitro. These results suggest that up-regulation of histamine H1 receptor expression by platelet-derived growth factor plays an important role in the initiation and progression of cardiovascular diseases.
血清和动脉组织中的组胺会促进动脉粥样硬化的发病机制以及冠状动脉血管痉挛的形成。由于组胺在特定部位的作用将由其特定受体介导，我们通过原位杂交和Northern印迹分析，研究了人组胺H1受体mRNA在动脉壁、培养的人主动脉内膜平滑肌细胞（SMC）、永生化SMC（ISS10）和内皮细胞（SE1）中的表达及定位。原位杂交显示，SMC和内皮细胞在体内表达H1受体mRNA，并且在主动脉和冠状动脉粥样硬化病灶增厚内膜中的SMC中表达增加。通过Northern印迹分析，我们还在培养的SMC、ISS10和SE1中检测到组胺H1受体mRNA，并发现血小板衍生生长因子在体外刺激SMC增加其mRNA的表达。这些结果表明，血小板衍生生长因子对组胺H1受体表达的上调在心血管疾病的发生和发展中起重要作用。
