Fukui H, Fujimoto K, Mizuguchi H, Sakamoto K, Horio Y, Takai S, Yamada K, Ito S
Department of Pharmacology II, Faculty of Medicine, Osaka University, Suita, Japan.
Biochem Biophys Res Commun. 1994 Jun 15;201(2):894-901. doi: 10.1006/bbrc.1994.1786.
The human histamine H1 receptor gene, an intron-lacking gene, was isolated with bovine H1 receptor cDNA [Yamashita, M., Fukui, H., Sugama, K., Horio, Y., Ito, S., Mizuguchi, H., and Wada, H. (1991) Proc. Natl. Acad. Sci. USA 88, 11515-11519] used as a probe. The receptor protein deduced from the nucleotide sequence of this gene was composed of 487 amino acid residues with a calculated Mr of 55,781 and possessed characteristic properties of GTP binding protein-coupled receptors. At the 5' flanking region of the human H1 receptor gene, we located potential TATA box and CACCC sequences, AP1 binding site-like sequences, glucocorticoid responsive element-like sequences, and other binding sequences for inducers. Northern blot analysis showed that H1 receptor mRNAs visualized as two bands with 3.0-kilobase and 3.5-kilobase nucleotides were expressed in peripheral tissues such as placenta, lung, skeletal muscle, and kidney and that only one species with 3.5-kilobase nucleotides was present in the brain. H1 receptor mRNA was most abundant in the placenta. The human H1 receptor gene was mapped to the chromosome 3p25 by the fluorescence in situ hybridization method.
以牛H1受体cDNA[山下真一、福井浩、菅间康、堀尾洋、伊藤诚、水口博、和田浩(1991)《美国国家科学院院刊》88, 11515 - 11519]为探针，分离出了人组胺H1受体基因，该基因无内含子。从该基因核苷酸序列推导的受体蛋白由487个氨基酸残基组成，计算的Mr为55781，具有GTP结合蛋白偶联受体的特征性质。在人H1受体基因的5'侧翼区，我们定位到了潜在的TATA盒和CACCC序列、类AP1结合位点序列、类糖皮质激素反应元件序列以及其他诱导剂结合序列。Northern印迹分析表明，H1受体mRNA呈现为两条带，分别为3.0千碱基和3.5千碱基核苷酸，在胎盘、肺、骨骼肌和肾等外周组织中表达，而在脑中仅存在一种3.5千碱基核苷酸的mRNA。H1受体mRNA在胎盘中最为丰富。通过荧光原位杂交法将人H1受体基因定位到染色体3p25。
