一种用于快速初步检测结构逆转录病毒的联合检测方法。

A combined assay for the rapid preliminary detection of structural retroviruses.

作者信息

Erlick B J, Fuscaldo A A, West W, Fuscaldo K, Brodsky I

出版信息

Cancer Lett. 1978 Mar;4(3):163-9. doi: 10.1016/s0304-3835(78)94182-4.

Abstract

This report describes the use of equilibrium gradients, RNA dependent DNA polymerase assays and electron microscopy (EM) in a combined assay for the rapid preliminary detection of intact retroviruses in crude preparations. Positive combined assays of platelets from preleukemic patients corresponded with karyotypic abnormalities found in these patients. Reconstruction experiments with Rauscher Leukemia Virus added to buffer or disrupted mouse spleen demonstrated the ease of detecting 10(9) or greater particles/g crude tissue, and the effects of buffer or added protein.

摘要

本报告描述了在一项联合检测中使用平衡梯度、RNA依赖性DNA聚合酶检测和电子显微镜（EM）来快速初步检测粗制品中的完整逆转录病毒。白血病前期患者血小板的联合检测阳性结果与这些患者中发现的核型异常相对应。将劳氏肉瘤病毒添加到缓冲液或破碎的小鼠脾脏中进行的重建实验表明，很容易检测到每克粗组织中有10⁹个或更多的病毒颗粒，以及缓冲液或添加蛋白质的影响。