Anchisi C, Batetta B, Sanna F, Fadda A M, Maccioni A M, Dessi S
Dipartimento Farmaco Chimico Tecnologico, Facoltà di Farmacia, Università degli Studi di Cagliari, Italy.
J Pharm Biomed Anal. 1995 Jan;13(1):65-71. doi: 10.1016/0731-7085(94)e0026-w.
Previous studies from the authors' laboratories have shown that cancer patients are characterized by lower levels of high-density lipoprotein cholesterol (HDL-C) compared with those of normal subjects. HDLs are a complex class of lipoproteins which can be divided mainly into two categories, HDL2 and HDL3, that have not only different lipid and protein composition but also different functions. Therefore, for a better understanding of the metabolism of HDL during tumour growth, the different subfractions of HDL (HDL2 and HDL3) were analysed in the serum of neoplastic patients using a rapid and simple high-performance liquid chromatography (HPLC) method for the analysis. The results obtained showed that serum from neoplastic patients exhibits a peculiar pattern in the distribution of HDL subfractions, consisting of a sharp decrease in HDL3 and a consequent increase of the normal HDL2/HDL3 ratio. It is suggested that evaluation of the HDL subfractions may be of clinical relevance for cancer status and that due to its simplicity, short analytical time and small sample volume required, the HPLC technique used in this study can be easily applied to routine analysis in cancer patients.
作者实验室先前的研究表明，与正常受试者相比，癌症患者的高密度脂蛋白胆固醇（HDL-C）水平较低。HDL是一类复杂的脂蛋白，主要可分为HDL2和HDL3两类，它们不仅具有不同的脂质和蛋白质组成，而且功能也不同。因此，为了更好地了解肿瘤生长过程中HDL的代谢情况，使用一种快速简单的高效液相色谱（HPLC）分析方法，对肿瘤患者血清中的HDL不同亚组分（HDL2和HDL3）进行了分析。所得结果表明，肿瘤患者血清中HDL亚组分的分布呈现出一种特殊模式，即HDL3急剧下降，导致正常的HDL2/HDL3比值升高。有人认为，HDL亚组分的评估可能与癌症状态具有临床相关性，并且由于本研究中使用的HPLC技术操作简单、分析时间短且所需样本量小，因此可以很容易地应用于癌症患者的常规分析。
