Kiyoi H, Fukutani H, Kubo K, Ohno R, Naoe T
Department of Medicine, Nagoya University School of Medicine, Aichi.
Intern Med. 1994 Dec;33(12):786-9. doi: 10.2169/internalmedicine.33.786.
We sequentially analyzed the immunoglobulin heavy chain (IgH) variable region gene of leukemia cells obtained from a chronic myeloid leukemia (CML) patient who had three episodes of B-lymphoid crisis after bone marrow transplantation. Southern blots using the JH probe showed a single rearranged band which differed at each crisis, although the rearranged bands of the BCR gene were the same at each crisis. The IgH variable region sequences of the leukemia cells at each crisis were different. These observations suggested that multiple clones were generated from the progenitor cells of the blast crisis, which were transformed at a very early stage of B-lymphocyte ontogeny.
我们对一名慢性髓性白血病（CML）患者骨髓移植后发生三次B淋巴细胞危象的白血病细胞免疫球蛋白重链（IgH）可变区基因进行了序列分析。使用JH探针的Southern印迹显示，每次危象均出现一条重排带，且各次危象的重排带不同，尽管BCR基因的重排带在每次危象时相同。每次危象时白血病细胞的IgH可变区序列均不同。这些观察结果提示，原始细胞危象的祖细胞产生了多个克隆，这些克隆在B淋巴细胞个体发育的非常早期阶段发生了转化。
