小鼠4号染色体上逆转录病毒限制位点Fv1周围区域的遗传图谱。

Genetic map of the region surrounding the retrovirus restriction locus, Fv1, on mouse chromosome 4.

作者信息

Stoye J P, Kaushik N, Jeremiah S, Best S

机构信息

National Institute for Medical Research, Ridgeway, Mill Hill, London, UK.

出版信息

Mamm Genome. 1995 Jan;6(1):31-6. doi: 10.1007/BF00350890.

DOI:10.1007/BF00350890

PMID:7719023

Abstract

The Friend virus susceptibility-1 (Fv1) gene maps to mouse Chromosome (Chr) 4 close to a cluster of four endogenous murine leukemia viruses (MLVs). To investigate the feasibility of cloning Fv1 by a positional approach, we have performed an extensive genetic analysis of this region of Chr 4. We have typed 368 backcross mice for the four proviruses, Nppa, Lck, and D4Smh6b. Recombinant animals were screened in a hierarchical fashion with a variety of other markers, including Fv1 and the isozyme marker Gpd1. A detailed genetic map of the region surrounding Fv1 was derived. Three markers, Xmv9, Nppa, and Iap3rc11, were identified that showed no recombination with Fv1. By combining backcross and recombinant inbred strain data, we estimated that Xmv9 and Nppa must lie within 0.6 cM of one another and Fv1.

摘要

Friend病毒易感性-1（Fv1）基因定位于小鼠4号染色体（Chr）上，靠近一组四种内源性鼠白血病病毒（MLV）。为了研究通过定位方法克隆Fv1的可行性，我们对4号染色体的该区域进行了广泛的遗传分析。我们对368只回交小鼠进行了四种前病毒Nppa、Lck和D4Smh6b的分型。用包括Fv1和同工酶标记Gpd1在内的多种其他标记，以分层方式筛选重组动物。得出了Fv1周围区域的详细遗传图谱。鉴定出三个标记Xmv9、Nppa和Iap3rc11，它们与Fv1没有重组。通过结合回交和重组近交系数据，我们估计Xmv9和Nppa彼此之间以及与Fv1的距离必须在0.6 cM以内。