Phillips R, Mawhinney T, Harmata M, Smith D
Department of Biochemistry, University of Missouri, Columbia 65212, USA.
Artif Cells Blood Substit Immobil Biotechnol. 1995;23(1):63-79. doi: 10.3109/10731199509117668.
Soluble A antigens and an enzyme-linked immunosorbent assay (ELISA) using type A2 erythrocyte membranes were used to study the activity of an alpha-N-acetyl-galactosaminidase from Gallus domesticus (domestic chicken). The enzyme readily hydrolyzed the terminal N-acetyl-alpha-D-galactosamine of the A antigen under a variety of conditions, converting it to H antigen. Conversion of the A antigen to H antigen produces blood type O, which is universally transfusable. These preliminary studies are important in determining optimal conditions for enzymatic conversion of blood type A to O if efficient large scale production of enzymatically converted, universally transfusable red blood cells is to be achieved.
利用可溶性A抗原以及一种使用A2型红细胞膜的酶联免疫吸附测定法（ELISA）来研究家鸡（家鸡）α-N-乙酰半乳糖胺酶的活性。该酶在多种条件下都能轻易水解A抗原的末端N-乙酰-α-D-半乳糖胺，将其转化为H抗原。A抗原转化为H抗原会产生O型血，O型血可通用输血。如果要实现高效大规模生产经酶转化的、可通用输血的红细胞，这些初步研究对于确定将A型血酶促转化为O型血的最佳条件至关重要。
