Ye B, Hadjmohammadi M, Khaledi M G
Department of Chemistry, North Carolina State University, Raleigh 27695.
J Chromatogr A. 1995 Feb 10;692(1-2):291-300. doi: 10.1016/0021-9673(94)01027-c.
Electrophoretic mobilities and capacity factors for a group of Trp-containing small peptides were determined by micellar electrokinetic chromatography (MEKC) using mixtures of a fluorocarbon anionic surfactant, lithium perfluorooctane sulfonate, and a hydrocarbon anionic surfactant, lithium dodecyl sulfate. Upon mixing these two surfactants, which have different microenvironments and interactive characteristics, greater control over migration of solutes is achieved. The changes in the composition of mixed micelles such as the mole fraction of the surfactants result in different solute-micelle binding as well as migration times of the micelles (tmc). Consequently, capacity factor, selectivity and elution window (tmc/t0) change with the composition of the mixed micellar system. Another characteristic of the mixtures of fluorocarbon-hydrocarbon surfactants is the possibility of forming two different types of micelles which offers an additional partitioning process for each solute in the MEKC system. Such a unique phenomenon offers a higher degree of selectivity control. This mixed MEKC system is quite effective for the separation of small peptides. It provides an alternative to the free-solution capillary zone electrophoresis system for the separation of charged solutes with nearly identical electrophoretic mobility.
使用含氟阴离子表面活性剂全氟辛烷磺酸锂和烃类阴离子表面活性剂十二烷基硫酸锂的混合物，通过胶束电动色谱法（MEKC）测定了一组含色氨酸的小肽的电泳迁移率和容量因子。将这两种具有不同微环境和相互作用特性的表面活性剂混合后，可以更好地控制溶质的迁移。混合胶束组成的变化，如表面活性剂的摩尔分数，会导致不同的溶质-胶束结合以及胶束的迁移时间（tmc）。因此，容量因子、选择性和洗脱窗口（tmc/t0）会随着混合胶束体系的组成而变化。碳氟化合物-烃类表面活性剂混合物的另一个特点是有可能形成两种不同类型的胶束，这为MEKC系统中的每种溶质提供了额外的分配过程。这种独特的现象提供了更高程度的选择性控制。这种混合MEKC系统对小肽的分离非常有效。它为自由溶液毛细管区带电泳系统提供了一种替代方法，用于分离电泳迁移率几乎相同的带电溶质。
