Lee K T, Sheen P C
Department of Surgery, Kaohsiung Medical College Hospital, Taiwan.
Dig Dis Sci. 1995 Apr;40(4):757-62. doi: 10.1007/BF02064976.
A panel of 12 biotinylated lectins was used to investigate the diversity of glycoconjugate on the epithelium of stone-containing intrahepatic bile ducts and compared to controls. Among the 12 lectins, only WGA (wheat germ agglutinin) and Con A (concanavalin agglutinin) stained the epithelium of stone-containing intrahepatic ducts. Con A, a glucose/mannose-specific lectin, bound weakly on the epithelium of the stone-bearing intrahepatic duct in 10 of the 25 specimens, but none of the controls. All stone-containing intrahepatic bile ducts were stained heavily and homogenously by WGA, the N-acetylglucosamine-specific lectin. The high columnar epithelia of both intramural and extramural glands were stained in the supranuclear region, while the serous acini of extramural glands were stained in whole cytoplasm. The epithelium of intrahepatic ducts from the controls was stained weakly by WGA only. The WGA receptors were not abolished by pretreatment of neuraminidase. This led us to conclude that the stone-containing intrahepatic ducts were rich in N-acetylglucosamine and the heavy and homogenous staining with WGA will be indicative of hypersecretion of mucus from stone-bearing intrahepatic bile ducts.
使用一组12种生物素化凝集素来研究含结石的肝内胆管上皮上糖缀合物的多样性，并与对照组进行比较。在这12种凝集素中，只有WGA（麦胚凝集素）和Con A（伴刀豆球蛋白A）能使含结石的肝内胆管上皮染色。Con A是一种对葡萄糖/甘露糖具有特异性的凝集素，在25个标本中的10个标本中，它在含结石的肝内胆管上皮上结合较弱，但对照组均未出现这种情况。所有含结石的肝内胆管均被WGA（一种对N-乙酰葡糖胺具有特异性的凝集素）强烈且均匀地染色。壁内和壁外腺体的高柱状上皮在核上区被染色，而壁外腺体的浆液性腺泡在整个细胞质中被染色。对照组肝内胆管上皮仅被WGA轻度染色。神经氨酸酶预处理并未消除WGA受体。这使我们得出结论，含结石的肝内胆管富含N-乙酰葡糖胺，并且WGA的强烈且均匀染色将表明含结石的肝内胆管黏液分泌过多。
