Gueth-Hallonet C, Santa-Maria A, Verroust P, Maro B
Département de Biologie du Développement, Institut Jacques Monod, CNRS-Université Paris VII, France.
Development. 1994 Nov;120(11):3289-99. doi: 10.1242/dev.120.11.3289.
During preimplantation development of the mouse embryo, a layer of outer cells differentiates into a perfect epithelium, the trophectoderm. The divergence between the trophectoderm and the inner cell mass takes place from the 8-cell stage to the 64-cell stage and precedes their commitment at the blastocyst stage. In this work, we have investigated the expression of gp330, a 330 x 10(3) M(r) glycoprotein found in clathrin-coated areas of the plasma membrane of some epithelial cells characterized by a high level of endocytic activity. Our results show that gp330 is first synthesized in 16-cell stage embryos and that its appearance is restricted to outer cells until the blastocyst stage. Furthermore, its expression is repressed in inner cells at a post-transcriptional level, probably through the development of extensive cell-cell contacts.
在小鼠胚胎植入前发育过程中，一层外层细胞分化形成完美的上皮组织，即滋养外胚层。滋养外胚层与内细胞团之间的分化发生在8细胞期至64细胞期，并先于它们在囊胚期的特化。在这项研究中，我们研究了gp330的表达，gp330是一种330×10³ 分子量的糖蛋白，存在于一些具有高水平内吞活性的上皮细胞质膜的网格蛋白包被区域。我们的结果表明，gp330在16细胞期胚胎中首次合成，其出现仅限于外层细胞，直至囊胚期。此外，其在内层细胞中的表达在转录后水平受到抑制，可能是通过广泛的细胞间接触的形成。
