Yamaguchi M, Imai T, Maeda T, Sakata M, Miyake A, Linzer D I
Department of Obstetrics and Gynecology, Osaka University Medical School, Japan.
Endocrinology. 1995 May;136(5):2040-6. doi: 10.1210/endo.136.5.7720652.
To identify factors that regulate proliferin (PLF) and PLF-related protein (PRP) secretion by the mouse placenta, placental cells from day 9 of pregnancy were cultured for up to 5 days, and PLF and PRP release into the medium was assessed by RIA. Transforming growth factor-alpha, interleukin-1 alpha, and interleukin-6 did not regulate either PLF or PRP secretion. However, treatment of primary placental cell cultures with 8-bromo-cAMP, cholera toxin, or forskolin resulted in 2- to 3-fold increases in the percentages of PLF- and PRP-producing cells in the population and corresponding increases in both PLF and PRP messenger RNA and secreted protein. The increase in the number of PLF-producing cells was accompanied by an increase in the number of cells expressing both PLF and mouse placental lactogen-I. These data suggest that cAMP levels can regulate trophoblast giant cell differentiation and, consequently, the amount of PLF and PRP secretion.
为了确定调节小鼠胎盘分泌增殖蛋白（PLF）和增殖蛋白相关蛋白（PRP）的因素，将妊娠第9天的胎盘细胞培养长达5天，并通过放射免疫分析评估PLF和PRP释放到培养基中的情况。转化生长因子-α、白细胞介素-1α和白细胞介素-6均不调节PLF或PRP的分泌。然而，用8-溴-cAMP、霍乱毒素或福斯可林处理原代胎盘细胞培养物，导致群体中产生PLF和PRP的细胞百分比增加2至3倍，同时PLF和PRP信使核糖核酸及分泌蛋白相应增加。产生PLF的细胞数量增加的同时，表达PLF和小鼠胎盘催乳素-I的细胞数量也增加。这些数据表明，环磷酸腺苷（cAMP）水平可调节滋养层巨细胞分化，从而调节PLF和PRP的分泌量。
