Karino S, Minegishi T, Ohyama Y, Tano M, Nakamura K, Miyamoto K, Tanaka S, Ibuki Y
Department of Obstetrics and Gynecology, Gunma University School of Medicine, Japan.
FEBS Lett. 1995 Apr 3;362(2):147-50. doi: 10.1016/0014-5793(95)00231-w.
In a previous experiment, it was shown that midkine (MK) was quite abundant in the follicular fluid; the concentration of MK in bovine follicular fluid was estimated to be 125 micrograms/l. To investigate the regulation of MK production in the ovary, we examined the effect of pregnant mare's serum gonadotropin (PMSG) and PMSG-hCG treatment on the expression of MK in rat ovary. The mRNA of midkine (MK) was increased by PMSG injection and decreased by PMSG and hCG injection; the profile of change of mRNA of MK was similar to that of FSH receptor. Using in situ hybridization, we observed that the MK mRNA localized to granulosa cells. These results suggest that the granulosa cells produce MK under the control of gonadotropin.
在先前的一项实验中，已表明中期因子（MK）在卵泡液中含量相当丰富；牛卵泡液中MK的浓度估计为125微克/升。为了研究卵巢中MK产生的调节机制，我们检测了孕马血清促性腺激素（PMSG）和PMSG - hCG处理对大鼠卵巢中MK表达的影响。注射PMSG可使中期因子（MK）的mRNA增加，而注射PMSG和hCG则使其减少；MK mRNA的变化模式与促卵泡激素（FSH）受体的相似。通过原位杂交，我们观察到MK mRNA定位于颗粒细胞。这些结果表明，颗粒细胞在促性腺激素的控制下产生MK。
