编码人脑中γ-氨基丁酸转氨酶的cDNA的筛选与序列测定。
Screening and sequence determination of a cDNA encoding the human brain 4-aminobutyrate aminotransferase.
作者信息
Osei Y D, Churchich J E
机构信息
Department of Biochemistry, University of Tennessee, Knoxville 37996-0840, USA.
出版信息
Gene. 1995 Apr 3;155(2):185-7. doi: 10.1016/0378-1119(94)00858-p.
PMID:7721088
Abstract
A human brain cDNA library constructed in the lambda ZAP II vector was screened using a fragment of pig brain cDNA encoding 4-aminobutyrate aminotransferase (pGaba-t). A cDNA that encodes the human brain Gaba-t (hGaba-t) has been isolated from the library and sequenced. Using the GenBank and EMBL databases, comparison of the predicted amino-acid sequence of hGaba-t with the pig enzyme revealed 95.4% homology.
摘要
使用编码4-氨基丁酸转氨酶（pGaba-t）的猪脑cDNA片段筛选构建于λZAP II载体中的人脑cDNA文库。从该文库中分离出了编码人脑Gaba-t（hGaba-t）的cDNA并进行了测序。利用GenBank和EMBL数据库，将hGaba-t预测的氨基酸序列与猪的该酶进行比较，发现同源性为95.4%。
Zotero 插件