Calderon I, Barak M, Abramovici H, Gruener N, Yavez H, Paz G, Homonnai Z T
Biochemistry Research Unit, Carmel Medical Center, Haifa, Israel.
J Androl. 1994 Nov-Dec;15(6):603-7.
Azoospermia is the cause of infertility in 8% of infertile male patients. Ten percent of those patients suffer from agenesis of the seminal vesicle (SV) and vas deferens (VD) agenesis. Currently, the diagnosis of SV and VD agenesis is based on low semen volume, low pH, and low fructose content of the seminal fluid of azoospermic men who have normal serum gonadotropins. In this study, an SV-specific sperm-coating antigen, the MHS-5 antigen, was used as a marker for the presence of SVs. The SV-specific protein (SVSP), MHS-5, was present in the control group but was not found in any of the seven samples from azoospermic men with proven agenesis of SV and VD. Another semen component, the prostate-specific antigen (PSA), whose presence in the semen is not influenced by the SV and VD agenesis, was found in both the study and the control groups. Its presence ruled out the possibility of azoospermia due to ejaculatory duct obstruction. The absence of MHS-5 antigen in seminal fluid can be used as a tool for a reliable diagnosis of agenesis of SV and VD in azoospermic men.
无精子症是8%不育男性患者不孕的原因。其中10%的患者患有精囊（SV）发育不全和输精管（VD）发育不全。目前，SV和VD发育不全的诊断基于精液量少、pH值低以及血清促性腺激素正常的无精子症男性精液中果糖含量低。在本研究中，一种SV特异性精子包被抗原，即MHS-5抗原，被用作SV存在的标志物。SV特异性蛋白（SVSP）MHS-5在对照组中存在，但在7例已证实患有SV和VD发育不全的无精子症男性的样本中均未发现。另一种精液成分，前列腺特异性抗原（PSA），其在精液中的存在不受SV和VD发育不全的影响，在研究组和对照组中均有发现。其存在排除了因射精管阻塞导致无精子症的可能性。精液中MHS-5抗原的缺失可作为一种可靠诊断无精子症男性SV和VD发育不全的工具。
