Bergantino E, Dainese P, Cerovic Z, Sechi S, Bassi R
Dipartimento di Biologia, Università di Padova, Italy.
J Biol Chem. 1995 Apr 14;270(15):8474-81. doi: 10.1074/jbc.270.15.8474.
The resistance of maize plants to cold stress has been associated with the appearance of a new chlorophyll a/b binding protein in the thylakoid membrane following chilling treatment in the light. The cold-induced protein has been isolated, characterized by amino acid sequencing, and pulse labeled with radioactive precursors, showing that it is the product of post-translational modification by phosphorylation of the minor chlorophyll a/b protein CP29 rather than the product of a cold-regulated gene or an unprocessed CP29 precursor. We show here that the CP29 kinase activity displays unique characteristics differing from previously described thylakoid kinases and is regulated by the redox state of a quinonic site. Finally, we show that maize plants unable to perform phosphorylation have enhanced sensitivity to cold-induced photoinhibition.
玉米植株对冷胁迫的抗性与光照下冷处理后类囊体膜中出现的一种新的叶绿素a/b结合蛋白有关。这种冷诱导蛋白已被分离出来，通过氨基酸测序进行了表征，并用放射性前体进行了脉冲标记，结果表明它是次要叶绿素a/b蛋白CP29经磷酸化后翻译后修饰的产物，而不是冷调节基因的产物或未加工的CP29前体。我们在此表明，CP29激酶活性具有与先前描述的类囊体激酶不同的独特特征，并受醌位点氧化还原状态的调节。最后，我们表明无法进行磷酸化的玉米植株对冷诱导的光抑制敏感性增强。
