Yu G, Deschenes R J, Fassler J S
Genetics Ph.D. Program, University of Iowa, Iowa City 52242, USA.
J Biol Chem. 1995 Apr 14;270(15):8739-43. doi: 10.1074/jbc.270.15.8739.
In a search for mutants exhibiting altered activity of the yeast transcription factor, Mcm1, we have identified the SLN1 gene, whose product is highly related to bacterial two-component sensor-regulator proteins. sln1 alleles identified in our screen increased Mcm1p-mediated transcriptional activation, while deletion of the SLN1 locus severely reduced Mcm1p activity. Our data establish that Mcm1p is a downstream target of the Sln1 signaling pathway. Yeast Sln1p was recently shown to be involved in osmoregulation and to depend on the Hog1 MAP kinase (Maeda, T., Wurgler-Murphy, S., and Saito, H. (1994) Nature 369, 242-245). We show that SLN1-mediated regulation of Mcm1p activity is independent of the Hog1 MAP kinase, and suggest that the role of SLN1 is not restricted to osmoregulation.
在寻找表现出酵母转录因子Mcm1活性改变的突变体的过程中，我们鉴定出了SLN1基因，其产物与细菌双组分传感调节蛋白高度相关。我们在筛选中鉴定出的sln1等位基因增强了Mcm1p介导的转录激活，而SLN1基因座的缺失则严重降低了Mcm1p的活性。我们的数据表明，Mcm1p是Sln1信号通路的下游靶点。酵母Sln1p最近被证明参与渗透调节，并依赖于Hog1丝裂原活化蛋白激酶（前田，T.，伍格勒-墨菲，S.，和斋藤，H.（1994年）《自然》369卷，242 - 245页）。我们表明，SLN1介导的对Mcm1p活性的调节独立于Hog1丝裂原活化蛋白激酶，并提出SLN1的作用不限于渗透调节。
