Stefano G B, Rodriguez M, Glass R, Cesares F, Hughes T K, Bilfinger T V
Department of Surgery, University Medical Center, State University of New York at Stony Brook 11794-8191, USA.
J Cardiovasc Surg (Torino). 1995 Feb;36(1):25-30.
The trauma of cardiopulmonary by-pass (CPB) in cardiac surgery results in a whole body diffuse inflammatory response characterized in part by hyperstimulation of leukocytes. Partially this is due to an increase in the release of biological response modifiers such as cytokines, as noted by the immunocyte stimulatory actions of cell-free plasma obtained postoperatively from CPB patients. The present study was conducted to determine whether CPB plasma induced immunocyte hyperstimulation can be prevented with naturally occurring immune inhibitory substances, specifically, interleukin (IL)-10 and/or morphine.
Controlled in vitro study of the application of drugs to naive immunocytes to block the exitation caused by CPB-plasma.
University-based tertiary care hospital.
Plasma was obtained from ten patients undergoing CPB. Eligibility included admission for elective cardiac surgery, which no chronic illnesses or acute processes.
Monocytes and granulocytes were pretreated with IL-10 and/or morphine before exposure to plasma obtained from patients undergoing CPB, as CPB-plasma would stimulate naive monocytes and granulocytes in a manner similar to that previously reported in CPB-patients.
Computer-assisted microscopic image analysis, measuring cellular conformational and velocity changes, was used to evaluate the effect of treatment on the immunocytes response to stimulation with CPB-plasma.
Pretreatment of cells with IL-10 and/or morphine significantly diminished the hyperstimulation induced by CPB-plasma in a concentration-dependent manner. In contrast, when the cells were initially or simultaneously exposed to CPB-plasma, IL-10 and/or morphine had no effect.
心脏手术中的体外循环（CPB）创伤会引发全身弥漫性炎症反应，其部分特征为白细胞过度刺激。部分原因是生物反应调节剂如细胞因子的释放增加，这从CPB患者术后获得的无细胞血浆的免疫细胞刺激作用中可以看出。本研究旨在确定CPB血浆诱导的免疫细胞过度刺激是否可以用天然存在的免疫抑制物质，特别是白细胞介素（IL）-10和/或吗啡来预防。
对未接触过的免疫细胞应用药物以阻断CPB血浆引起的兴奋的体外对照研究。
大学附属三级护理医院。
从10名接受CPB的患者中获取血浆。入选标准包括择期心脏手术入院，且无慢性疾病或急性病症。
单核细胞和粒细胞在接触CPB患者的血浆之前先用IL-10和/或吗啡进行预处理，因为CPB血浆会以类似于先前在CPB患者中报道的方式刺激未接触过的单核细胞和粒细胞。
使用计算机辅助显微镜图像分析，测量细胞形态和速度变化，以评估治疗对免疫细胞对CPB血浆刺激反应的影响。
用IL-10和/或吗啡对细胞进行预处理以浓度依赖的方式显著减轻了CPB血浆诱导的过度刺激。相比之下，当细胞最初或同时暴露于CPB血浆时，IL-10和/或吗啡没有效果。
