Riley P J, Byford R L, Hallford D M, Campbell J W, Perez-Eguia E
Department of Entomology, Plant Pathology and Weed Science, New Mexico State University, Las Cruces 88003, USA.
J Econ Entomol. 1995 Apr;88(2):320-5. doi: 10.1093/jee/88.2.320.
Nine yearling crossbred beef steers, Bos taurus L., were used to examine physiological responses to Gulf Coast tick, Amblyomma maculatum Koch, infestation. Steers were stanchioned indoors in individual environmentally controlled rooms. On day 0, each animal received 0, 25, or 75 pairs of ticks. Physiological variables measured daily were feed intake, heart rates, rectal temperatures, and respiration rates. Blood samples were collected from each animal on days 7, 21, and 42 for serum constituent analysis. To monitor metabolic hormone status, intensive blood samples were collected hourly for 6 h on days 21 and 42. Throughout the treatment period, feed intake values were similar among treatments resulting in comparable body weight at the end of the trial. Heart rates and rectal temperatures were unaffected, however, respiration rates of steers infested with 25 pairs of ticks were higher than the other treatment groups. Treatment effects were detected in uric acid concentrations on day 7 in steers infested with 75 pairs of ticks. Treatment effects were detected in total, direct and indirect bilirubin, gamma-glutamyl transpeptidase, and aspartate amino transferase concentrations. Likewise, creatine kinase concentrations were higher in the tick-infested steers on day 7. Elevated white blood cell counts were observed in tick-infested steers. All other serum components were similar and were within their normal ranges. Serum insulin, prolactin, growth hormone, and cortisol concentrations were unaffected by tick infestations. Gulf coast tick infestation resulted in altered blood composition indicative of infection caused by tick feeding habits.
选用9头杂种一岁公牛（Bos taurus L.）来研究其对墨西哥湾沿岸蜱（Amblyomma maculatum Koch）感染的生理反应。将公牛拴系在室内各自的环境控制房间中。在第0天，每头动物分别接种0、25或75对蜱。每天测量的生理变量包括采食量、心率、直肠温度和呼吸频率。在第7天、21天和42天从每头动物采集血样用于血清成分分析。为监测代谢激素状态，在第21天和42天每小时采集密集血样，持续6小时。在整个治疗期间，各处理组的采食量相似，试验结束时体重相当。心率和直肠温度未受影响，然而，接种25对蜱的公牛的呼吸频率高于其他处理组。在接种75对蜱的公牛中，第7天的尿酸浓度检测到处理效应。在总胆红素、直接胆红素、间接胆红素、γ-谷氨酰转肽酶和天冬氨酸氨基转移酶浓度方面检测到处理效应。同样，第7天接种蜱的公牛的肌酸激酶浓度较高。在接种蜱的公牛中观察到白细胞计数升高。所有其他血清成分相似且在正常范围内。血清胰岛素、催乳素、生长激素和皮质醇浓度不受蜱感染的影响。墨西哥湾沿岸蜱感染导致血液成分改变，表明由蜱的摄食习性引起感染。
