血型Lewis特异性糖脂的酶促合成。

Enzymatic synthesis of blood-group Lewis-specific glycolipids.

作者信息

Prohaska R, Schenkel-Brunner H, Tuppy H

出版信息

Eur J Biochem. 1978 Mar;84(1):161-6. doi: 10.1111/j.1432-1033.1978.tb12152.x.

DOI:10.1111/j.1432-1033.1978.tb12152.x

Abstract

A blood-group Lewis precursor glycolipid was isolated from the plasma of a Lewis-negative individual [Le(a--b--)] and treated with fucosyltransferases from human gastric mucosa and GDP-fucose. Subsequently the glycolipid was adsorbed onto Le(a--b--) erythrocytes and the presence of blood-group Lewis antigens was assessed by passive hemagglutination with anti-Lewis sera. It was shown that the precursor glycolipid was enzymatically transformed to blood-group Lewis a (Lea) and Lewis b (Leb) specific glycolipids. Leb-glycolipid was also synthesized by fucosylation of an isolated Lea-glycolipid. Moreover Le(a--b--) erythrocytes were shown to develop Lea and Leb activities when subjected to enzymatic fucosylation, thus showing that Lewis-negative cells carry blood-group Lewis precursor glycolipid on the surface of their membrane. Le(a + b--) erthrocytes, upon enzymatic fucosylation, acquired Leb activity.

摘要

从一名Lewis阴性个体[Le(a--b--)]的血浆中分离出一种血型Lewis前体糖脂，并用来自人胃黏膜的岩藻糖基转移酶和GDP-岩藻糖进行处理。随后，该糖脂吸附到Le(a--b--)红细胞上，并用抗Lewis血清通过被动血凝反应评估血型Lewis抗原的存在情况。结果表明，前体糖脂被酶促转化为血型Lewis a（Lea）和Lewis b（Leb）特异性糖脂。Leb-糖脂也可通过对分离出的Lea-糖脂进行岩藻糖基化合成。此外，Le(a--b--)红细胞在进行酶促岩藻糖基化时显示出产生Lea和Leb活性，从而表明Lewis阴性细胞在其细胞膜表面携带血型Lewis前体糖脂。Le(a + b--)红细胞在酶促岩藻糖基化后获得了Leb活性。