Mori M, Tsushima H, Matsuda T
Department of Pharmacology, Nagoya City University Medical School, Japan.
Jpn J Pharmacol. 1994 Dec;66(4):445-50. doi: 10.1254/jjp.66.445.
The effects of microinjection of purinoceptor agonists into the hypothalamic supraoptic nucleus (SON) on urination were examined in water-loaded and ethanol-anesthetized rats. Adenosine triphosphate (ATP), but neither adenosine diphosphate (ADP), adenosine monophosphate (AMP) nor adenosine, concentration-dependently decreased the urine outflow with concomitant increase in the urine osmotic pressure. The ED50 value for ATP was approx. 60 nmol. The antidiuretic effect of ATP was blocked either by prior injection of theophylline (an antagonist of the P1-type purinoceptor) or by intravenous administration d(CH2)5-D-Tyr(Et)-valine-arginine-vasopressin (VAVP). These results suggest that ATP injected into the SON has antidiuretic effects due to release of AVP through an activation of theophylline-sensitive purinoceptors.
在下丘脑视上核（SON）微量注射嘌呤受体激动剂对水负荷和乙醇麻醉大鼠排尿的影响进行了研究。三磷酸腺苷（ATP）可浓度依赖性地减少尿流出量，并伴随尿渗透压升高，而二磷酸腺苷（ADP）、一磷酸腺苷（AMP）和腺苷则无此作用。ATP的半数有效剂量（ED50）约为60 nmol。预先注射茶碱（P1型嘌呤受体拮抗剂）或静脉注射d(CH2)5-D-Tyr(Et)-缬氨酸-精氨酸-加压素（VAVP）均可阻断ATP的抗利尿作用。这些结果表明，注入SON的ATP通过激活对茶碱敏感的嘌呤受体释放血管加压素（AVP），从而产生抗利尿作用。
