Yamaguchi K, Carr B I, Nalesnik M A
Department of Transplant Surgery, University of Pittsburgh School of Medicine, Pennsylvania, USA.
J Surg Oncol. 1995 Apr;58(4):240-5. doi: 10.1002/jso.2930580409.
Single and double immunohistochemical staining for transforming growth factor (TGF)-alpha and epidermal growth factor-receptor (EGF-R) was done in order to identify the localization of TGF-alpha and EGF-R in human hepatocellular carcinoma (HCC). Single immunohistochemical staining for TGF-alpha showed immunoreactivity in the cytoplasm of hepatoma cells in 22 of 30 cases of HCC. The localization of TGF-alpha was heterogeneous from HCC cells to HCC cells. In the surrounding regenerative nodules, the hepatocytes were mildly to moderately positive for TGF-alpha. The proliferating bile ductules and peripheral nerves were also immunopositive for TGF-alpha. Single immunohistochemical staining for EGF-R demonstrated a linear localization of EGF-R along the cell membrane of the HCC cells in 21 of the 30 cases of HCC. In the regenerative nodules, the hepatocytes also showed linear staining along the cell membrane. Double staining for TGF-alpha and EGF-R in 12 cases of HCC showed a concurrent localization of TGF-alpha and EGF-R in some hepatoma cells and isolated localization of the two substances of other HCC cells. These combinations either abruptly moved around or intermingled with each other. These immunohistochemical results thus support the theory of an autocrine, paracrine, and endocrine mechanism of TGF-alpha and EGF-R on the proliferation of human hepatocellular carcinoma.
为了确定转化生长因子（TGF）-α和表皮生长因子受体（EGF-R）在人肝细胞癌（HCC）中的定位，进行了TGF-α和EGF-R的单重和双重免疫组织化学染色。TGF-α的单重免疫组织化学染色显示，在30例HCC中的22例中，肝癌细胞的细胞质中有免疫反应性。TGF-α在肝癌细胞之间的定位是异质性的。在周围的再生结节中，肝细胞对TGF-α呈轻度至中度阳性。增殖的胆小管和周围神经对TGF-α也呈免疫阳性。EGF-R的单重免疫组织化学染色显示，在30例HCC中的21例中，EGF-R沿肝癌细胞膜呈线性定位。在再生结节中，肝细胞也沿细胞膜呈线性染色。12例HCC中TGF-α和EGF-R的双重染色显示，在一些肝癌细胞中TGF-α和EGF-R同时定位，而在其他肝癌细胞中这两种物质单独定位。这些组合要么突然移动，要么相互交织。因此，这些免疫组织化学结果支持了TGF-α和EGF-R对人肝细胞癌增殖的自分泌、旁分泌和内分泌机制的理论。
