Nakashima N, Haji M, Sakai Y, Ono Y, Umeda F, Nawata H
Third Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Metabolism. 1995 Apr;44(4):543-8. doi: 10.1016/0026-0495(95)90065-9.
Dehydroepiandrosterone (DHEA) and its sulfate derivative (DHEA-S) reportedly have antidiabetic and antiobesity effects. The effect of DHEA on glucose uptake in cultured human fibroblasts was examined. Incubation of cells with supraphysiologic concentrations of DHEA (10(-5) mol/L) for > or = 10 hours enhanced 2-deoxyglucose (2-DG) uptake significantly (P < .05). Supraphysiologic concentrations of insulin (10(-7) mol/L) increased the sensitivity of glucose uptake to DHEA. Conversely, the sensitivity of glucose uptake to insulin was increased by incubating cells with 10(-6) mol/L DHEA. Both the abundance of transcripts encoding glucose transporter-1 (Glut-1) and the maximal velocity (Vmax) of 2-DG transport were increased in cultured fibroblasts incubated with DHEA. Cultured fibroblasts expressed a specific binding factor with low affinity for [3H]DHEA (maximal number of binding sites, 18,496 sites per cell; Kd, 298 nmol/L). Other androgen hormones exerted a less-marked effect on glucose uptake; DHEA-S had no effect. These results suggested that DHEA increases Glut-1 mRNA through binding to a specific factor in cultured human fibroblasts and thereby stimulates glucose uptake in these cells.
据报道，脱氢表雄酮（DHEA）及其硫酸酯衍生物（DHEA-S）具有抗糖尿病和抗肥胖作用。研究了DHEA对培养的人成纤维细胞葡萄糖摄取的影响。用超生理浓度的DHEA（10^(-5) mol/L）孵育细胞≥10小时可显著增强2-脱氧葡萄糖（2-DG）摄取（P <.05）。超生理浓度的胰岛素（10^(-7) mol/L）可增加葡萄糖摄取对DHEA的敏感性。相反，用10^(-6) mol/L DHEA孵育细胞可增加葡萄糖摄取对胰岛素的敏感性。在与DHEA孵育的培养成纤维细胞中，编码葡萄糖转运蛋白-1（Glut-1）的转录本丰度和2-DG转运的最大速度（Vmax）均增加。培养的成纤维细胞表达一种对[3H]DHEA亲和力低的特异性结合因子（最大结合位点数，每个细胞18496个位点；解离常数，298 nmol/L）。其他雄激素激素对葡萄糖摄取的作用较小；DHEA-S无作用。这些结果表明，DHEA通过与培养的人成纤维细胞中的特定因子结合来增加Glut-1 mRNA，从而刺激这些细胞中的葡萄糖摄取。
