McGeachin R B, Bailey C A
West Campus Library, Texas Agricultural Experiment Station, Texas A&M University System, College Station 77843, USA.
Poult Sci. 1995 Feb;74(2):407-11. doi: 10.3382/ps.0740407.
A normal phase HPLC method employing a hexane:ethyl acetate mobile phase solvent gradient for the simultaneous separation and quantification of carotenoid pigments and vitamins A and E has been developed. The gradient consists of a 10-min linear change from 1 to 2 mL/min and 95:5 to 70:30 hexane:ethyl acetate mobile phase, which is followed by 7 min of isocratic mobile phase of 70:30 hexane:ethyl acetate at 2 mL/min. The stationary phase consisted of a 4-mu silica column. A photodiode array detector generated simultaneous chromatogram data at three wavelengths (294, 325, and 445 nm) with broad spectrum data at peaks. The method produced good separation of trans-lutein and zeaxanthin.
已开发出一种正相高效液相色谱法，该方法采用己烷：乙酸乙酯流动相溶剂梯度，用于同时分离和定量类胡萝卜素色素以及维生素A和E。该梯度包括在10分钟内从1至2 mL/分钟线性变化，流动相从95:5己烷：乙酸乙酯变为70:30己烷：乙酸乙酯，随后在2 mL/分钟的流速下保持7分钟的70:30己烷：乙酸乙酯等度流动相。固定相由一根4μm硅胶柱组成。光电二极管阵列检测器在三个波长（294、325和445 nm）下生成同步色谱图数据，并在峰处生成宽谱数据。该方法实现了反式叶黄素和玉米黄质的良好分离。
