钙调蛋白是通过环ADP核糖触发的类兰尼碱受体钙通道介导钙离子诱导的钙离子释放的选择性介质。
Calmodulin is a selective mediator of Ca(2+)-induced Ca2+ release via the ryanodine receptor-like Ca2+ channel triggered by cyclic ADP-ribose.
作者信息
Tanaka Y, Tashjian A H
机构信息
Department of Molecular and Cellular Toxicology, Harvard School of Public Health, Harvard Medical School, Boston, MA 02115, USA.
出版信息
Proc Natl Acad Sci U S A. 1995 Apr 11;92(8):3244-8. doi: 10.1073/pnas.92.8.3244.
The ryanodine receptor-like Ca2+ channel (RyRLC) is responsible for Ca2+ wave propagation and Ca2+ oscillations in certain nonmuscle cells by a Ca(2+)-induced Ca2+ release (CICR) mechanism. Cyclic ADP-ribose (cADPR), an enzymatic product derived from NAD+, is the only known endogenous metabolite that acts as an agonist on the RyRLC. However, the mode of action of cADPR is not clear. We have identified calmodulin as a functional mediator of cADPR-triggered CICR through the RyRLC in sea urchin eggs. cADPR-induced Ca2+ release consisted of two phases, an initial rapid release phase and a subsequent slower release. The second phase was selectively potentiated by calmodulin which, in turn, was activated by Ca2+ released during the initial phase. Caffeine enhanced the action of calmodulin. Calmodulin did not play a role in inositol 1,4,5-trisphosphate-induced Ca2+ release. These findings offer insights into the multiple pathways that regulate intracellular Ca2+ signaling.
类兰尼碱受体钙通道（RyRLC）通过钙诱导钙释放（CICR）机制，负责某些非肌肉细胞中的钙波传播和钙振荡。环ADP核糖（cADPR）是一种由NAD+衍生的酶促产物，是已知的唯一一种对RyRLC起激动剂作用的内源性代谢物。然而，cADPR的作用模式尚不清楚。我们已经确定钙调蛋白是海胆卵中通过RyRLC介导cADPR触发的CICR的功能介质。cADPR诱导的钙释放包括两个阶段，一个是初始快速释放阶段，另一个是随后的较慢释放阶段。第二阶段被钙调蛋白选择性增强，而钙调蛋白又被初始阶段释放的钙激活。咖啡因增强了钙调蛋白的作用。钙调蛋白在肌醇1,4,5-三磷酸诱导的钙释放中不起作用。这些发现为调节细胞内钙信号的多种途径提供了见解。
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