Schwarzhoff R, Schwörer H, Fornefeld H, Morys-Wortmann C, Katsoulis S, Creutzfeldt W, Fölsch U R, Schmidt W E
I. Department of Medicine, Christian-Albrechts-University of Kiel, Germany.
Regul Pept. 1995 Jan 5;55(1):57-66. doi: 10.1016/0167-0115(94)00092-c.
The gut-brain neuropeptide pituitary adenylate cyclase activating polypeptide (PACAP) is a novel highly conserved member of the secretin-glucagon-VIP peptide family comprising 38 or 27 amino acid residues. In this study, we investigate the actions of PACAP 1-27 or PACAP 1-38 on jejunal and caecal muscle strips from pig or guinea pig and demonstrate the neutralizing effect of two PACAP-specific monoclonal antibodies of the IgG1 subtype, RSP27II and RSP38. These antibodies were used to set up assay systems specific for PACAP 1-27 or PACAP 1-38. Monoclonal antibody RSP27II recognizes exclusively PACAP 1-27, whereas RSP38 binds only PACAP 1-38. PACAP 1-27 and PACAP 1-38 relax taenia caeci dose-dependently in the presence of guanethidine and scopolamine. Both peptides inhibit the spontaneous contractions of porcine jejunal muscle strips equipotently. Monoclonal antibodies RSP27II and RSP38 specifically neutralize the actions of either exogenously applied or endogenously released PACAP. Thus, they represent processing-specific tools to examine the physiological role of both molecular forms of PACAP in the gastrointestinal tract.
肠-脑神经肽垂体腺苷酸环化酶激活多肽（PACAP）是促胰液素-胰高血糖素-VIP肽家族中一个新的高度保守成员，由38或27个氨基酸残基组成。在本研究中，我们研究了PACAP 1-27或PACAP 1-38对猪或豚鼠空肠和盲肠肌条的作用，并证明了两种IgG1亚型的PACAP特异性单克隆抗体RSP27II和RSP38的中和作用。这些抗体被用于建立针对PACAP 1-27或PACAP 1-38的检测系统。单克隆抗体RSP27II仅识别PACAP 1-27，而RSP38仅结合PACAP 1-38。在胍乙啶和东莨菪碱存在的情况下，PACAP 1-27和PACAP 1-38剂量依赖性地舒张盲肠带。两种肽均能同等程度地抑制猪空肠肌条的自发收缩。单克隆抗体RSP27II和RSP38能特异性中和外源性应用或内源性释放的PACAP的作用。因此，它们是用于研究PACAP两种分子形式在胃肠道中生理作用的加工特异性工具。
